›› 1993, Vol. 36 ›› Issue (3): 272-276.

• 研究论文 • 上一篇    下一篇

舞毒蛾核型多角体病毒亚致死剂量对舞毒蛾幼虫的影响

丁翠 马可   

  • 出版日期:1993-08-20 发布日期:1993-08-20

EFFECTS OF SUBLETHAL DOSES OF NPV ON LARVAEOF GYPSY MOTH

DING TSUEY MICHAEL MA   

  • Online:1993-08-20 Published:1993-08-20

摘要: 用舞毒蛾核型多角体病毒亚致死剂量1.3X104-1.3X106多角体/毫升对舞毒蛾(Lymantri dispar)五龄雌雄幼虫分别进行感染后发现,除获得死亡率的不同外,存活个体化蛹数及雌雄比例亦发生变化。雌雄比例倾向于雄性。电镜照片显示在雌性幼虫中被侵染的组织包括脂肪、气管基底膜、腹神经节、胸腺、卵巢及大脑,而胸腺、卵巢和大脑被核型多角体病毒感染的报道很少。使用免疫金颗粒标记技术测得细胞核中的舞毒蛾核型多角体病毒与其抗体间具有强烈的亲和性。

Abstract: Newly molted male and female 5th-instar larvae of gypsy moth, Lymantria dispar, were separately infected with a LDMNPV suspension of known concentration: 1.3× 104-106 PIB,/ml.The experimental results demonstrated that sublethal doses of LDMNPV not only caused dif ferent mortalities of male and female larvae,but also affected their pupal number and sex ratio which was in favor of male. The EM pictures of ultrasection showed that fat body,trachea, ganglia, brain, ovary and prothoracic gland of female moth were infected by LDMNPV. Little data about brain, ovary and prothorcic gland infected by NPV is available. Polyhedra existed in nuclei of infected cells display strong reaction with antibody of LDMNP using immunogold-labelled technique.

Key words: Lymantria dispar&mdash, &mdash, LDMNPV&mdash, &mdash, sex ratio