关键词: 蚕蛹, 雌雄鉴别, 近红外光谱, 模式识别

Abstract: In this paper, difference between male and female pupae of Bombyx mori in near infrared reflectance spectra(NIRS) and the feasibility and method of spectra recognition to identify the sex of fresh pupae were studied. By Model 6250 NIR instrument, indestructive determination of 721 fresh silkworm pupae selected from Chinese race, Japanese race and their hybrid was made with suitable wavelengthschosen between 680nm and 1235nm. Fifty male and fifty female pupae was randomly selected from 217 pupae of Japanese race as learning samples. The feature at 5 wavelengths was extracted as observation vector from data of their second derivative spectra by a stepwise discriminant analysis. Statistical analysis showed that there were extremely significant differences between male and female pupae in NIRS at the 5wavelengths. When the Bayes quadratic discriminant function built by the feature was used to predict the sex of the other 621 test samples, 98.7% of correct rate of the identification recognition was obtained. This method is much better than other identification ones which use weight or size of pupae as their feature. The mechanism is discussed.

Key words: silkworm (Bombyx mori) pupa, determination of pupa sex, NIRS, pattern recognition.