棉铃虫地理种群的等位酶变异

摘要/Abstract

摘要： 利用聚丙烯酰胺梯度凝胶电泳检测了棉铃虫Helicoverpa armigera的13种等位酶：α-磷酸甘油脱氢酶(α-GPDH)、酸性磷酸酯酶(ACPH)、碱性磷酸酯酶(ALP)、醛氧化酶(AO)、酯酶(EST)、谷氨酸草酰乙酸转氨酶(GOT)、己糖激酶(HEX)、亮氨酸氨肽酶(LAP)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、磷酸葡萄糖变位酶(PGM)和黄嘌呤脱氢酶(XDH)，染色采用双染法。对其中9种等位酶的遗传变异进行了分析，包括13个位点，6个位点表现出多态性，7个位点是单态的，其中多态性位点比例为46.15%。AO、GOT、LAP、LDH、ME和XDH计算出棉铃虫的平均杂合度为0.1160，南京、成都、武穴、衡阳和哈密5个种群的平均遗传距离为0.0008～0.0293，平均遗传相似度为0.9707～0.992。棉铃虫种群内存在很高的遗传多态性，而已测定的种群间遗传分化程度较小，种群间没有基因交流的障碍。迁飞阻碍了不同地理种群间的遗传分化。

关键词: 等位酶, 遗传变异, 棉铃虫

Abstract: Thirteen allozymes,α-glycerophosphate dehydrogenase (α-GPDH), acid phosphatase (ACPH), alkaline phosphatase (ALP), aldehyde oxidase (AO), esterase (EST), glutamate oxaloacetate transaminase (GOT), hexokinase (HEX), leucine aminopeptidase (LAP), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), malate enzyme (ME), phosphoglucomutase (PGM), xanthine dehydrogenase (XDH), were analysed by polyacrylamide gradient gel electrophoresis with double stain method. The genetic variations of 9 allozymes within and among 5 geographic populations were investigated. Among the 13 loci examined, 6 loci were polymorphic and 7 loci were monomorphic. The percentage of polymorphic loci was 46.15%. As calculated with the results of AO, GOT, LAP, LDH, ME and XDH, the average heterozygosity of Helicoverpa armigera was 0.1160.Among Nanjing, Chengdu, Wuxue , Hengyang and Hami populations, their mean genetic distance was 0.0008～0.0293 and mean genetic similarity was 0.9707～0.992. It means that there exits high genetic diversity in each population, but the genetic differentiation among these populations is very limited. There was no barrier of gene flow among these investigated populations. The migration could hamper more genetic differentiation among different geographic populations.

Key words: allozyme, genetic variation, Helicoverpa armigera (Hübner)

徐广，郭予元，吴孔明. 棉铃虫地理种群的等位酶变异[J]. , 2000, 43(-1): 63-69.

XU Guang，GUO Yu-yuan，WU Kong-ming. llozyme variations within and among five geographic populations of Helicoverpa armigera (Hübner)[J]. , 2000, 43(-1): 63-69.

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棉铃虫地理种群的等位酶变异

llozyme variations within and among five geographic populations of Helicoverpa armigera (Hübner)

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