›› 2002, Vol. 45 ›› Issue (2): 165-169.

• 研究论文 • 上一篇    下一篇

星天牛幼虫肠道木聚糖酶的纯化和性质

董亚敏,殷幼平,曹月青,何正波   

  • 出版日期:2002-04-20 发布日期:2002-04-20

Purification and properties of xylanase from Anoplophora chinensis (Forster)

DONG Ya-Min, YIN You-Ping1*, CAO Yue-Qing, HE Zheng-Bo   

  • Online:2002-04-20 Published:2002-04-20

摘要: 星天牛Anoplophora chinensis (Frster)幼虫肠道匀浆液经80%丙酮沉淀、Q-Sepharose阴离子交换柱层析、PAGE制备电泳等方法纯化后,获得在SDS-PAGE上呈现单一区带的木聚糖酶。该酶的分子量约25 kD,等电点约4.0,最适温度50℃,最适pH 5.4,pH 3.0~7.8对酶活性的恢复无大的影响, 50℃保温2 h仍有60%酶活性。Hg2+、MnO-4、变性剂SDS完全抑制该酶活性, Cu2+、Mn2+、Ag+、Zn2+、Pb+、脲对酶活性有强烈的抑制作用。该酶具有水解纤维素的交叉活性,其Km值为2.47 mg/mL,Vmax为0.6 IU/mL。

关键词: 星天牛, 木聚糖酶, 纯化, 性质

Abstract: A xylanase was purified from the homogenate of alimentary canal of Anoplophora chinensis (Froster) by acetone precipitation, Q-Sepharose ion-exchange chromatography and preparative PAGE. The xylanase had a molecular mass approximate 25 kD and pI 4.0. For the action of the enzyme isolated, the most optimal temperature and pH were 50℃ and 5.4 respectively. About 60% of the enzyme activity remained after incubating it at 50℃ for 2 hours. The enzyme could be inhabited by Hg2+,MnO-4,SDS, Cu2+, Mn2+, Ag+, Zn2+, Pb+ and Urea. It could hydrolyse carboxymethylcellulose (CMC). The enzyme had a Km of 2.47 mg/mL and a Vmax of 0.6 IU/mL for birchwood xylan at 50℃ and pH 4.4

Key words: Anoplophora chinensis (Forster), xylanase, purification, properties