›› 2002, Vol. 45 ›› Issue (4): 538-543.

• 研究论文 • 上一篇    下一篇


张民照, 康乐   

  • 出版日期:2002-08-20 发布日期:2002-08-20

Amplified fragment length polymorphism (AFLP) and its applications in entomological research

ZHANG Min-Zhao, KANG Le   

  • Online:2002-08-20 Published:2002-08-20

摘要: 扩增片段长度多态性(AFLP)是一种新兴的很有效的分子遗传标记方法, 它通过对基因组DNA限制性内切酶酶切片段进行选择性扩增而揭示多态性,具有快速、经济简便、不需要预先知道模板DNA的信息、模板需要量少、重复性高、结果可靠及具有很高的信息含量等优点。AFLP也具有缺点,主要是标记是显性的,同其他显性标记一样,不能区分杂合体和纯合体,因而不能更好地估算种群遗传的变异,对种群遗传结构的分析不能提供更多的统计信息;AFLP技术较复杂,而且经常使用放射性同位素,对模板DNA质量要求也较高。为了克服AFLP的这些缺点,人们又在其基础上发展了其他相关技术,例如AFRP、SAMPL、DALP和TE-AFLP等。目前AFLP在昆虫方面的应用还不是很多,处于初级阶段,主要应用在生态型鉴定、种群遗传分析、连锁图谱构建等方面,相信随着其技术的发展完善,必将会越来越多地应用于昆虫学的研究中。

关键词: 昆虫, AFLP, 分子遗传标记

Abstract: Amplified fragment length polymorphism (AFLP) is a new and powerful molecular genetic marker for DNA fingerprinting based on selective amplification of total DNA restriction fragments. AFLP is a fast and economic technique. It needs little template DNA for analysis and can generate fingerprints of any DNA without much prior sequence knowledge. Data produced by AFLP are reproducible, reliable and highly informative. However, AFLP has some disadvantages. AFLP markers are dominant, and thus can not distinguish heterogeneous loci from homologous loci. Therefore, they provide less precise estimates of population genetic variability and have limited statistical power for detecting population genetic structure. Technically, AFLP is quite complicated and often involves the use of radioactive isotopes. Also,AFLP requires the high quality template DNA. In order to overcome these limitations, some related and improved methods have been developed, such as AFRP, SAMPL, DALP and TE-AFLP. So far, the application of AFLP technique in entomology is in its infancy. It has been mainly used in the identification of biotypes, analyses of population genetics and construction of linkage genetic maps. With further improvement of this technique, it will be more widely employed in entomology.

Key words: insect, AFLP, molecular genetic marker