关键词: DNA提取, RAPDPCR, 昆虫, 标本, 分子系统学

Abstract: Genomic DNA were extracted and amplified using the polymerase chain reaction and arbitrary primers from specimens of seven insect species: Chrysomela vigintipunctata (Scopoli) (Coleptera: Chrycomelidae), Harmonia axyridis Pollas and Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae), Agrotis ypsilon (Rottemberg) (Lepidoptera: Noctuidae), Crocothemis servilia Drury (Odonata: Libellulidae), and Oxya abentata Willemse and O. chinensis (Thunberg) (Orthoptera: Catantopidae). Of the specimens assayed, some had been naturally dried, some oven-dried and the remainder had been preserved in alcohol. The results suggest that, with respect to DNA extraction, oven-dried specimens and those preserved in alchohol are better than naturally dried specimens. Extraction of DNA extraction using the CTAB method was easier, and more economical than other methods.

Key words: DNA extraction, RAPD-PCR, insect, specimen, molecular systematics