关键词: 不等单蚤, 缓慢细蚤, 成熟过程, 酶, 组织化学

Abstract: The distribution and activity of alkaline phosphatase (AKP), acid phosphatase (ACP) and adenosine triphosphatase (ATPase) in newly emerged and engorged adults of Monopsyllus anisus (Rothschild) and Leptopsylla segnis (Schonherr) were studied with histochemistry techniques and image analysis of microscope photographs. The results indicated that in newly emerged adults, alkaline phosphatase (AKP) and acid phosphatase (ACP) were mainly distributed in midgut, nerve nucleus, testis, ejaculatory duct, oviduct and spermathecal gland, while adenosine triphosp hatase (ATPase) was distributed in all tissues. After blood ingestion and digestion, the activity of three enzymes increased in midguts. In engorged adults, except that the activity of alkaline phosphatase (AKP) decreased 72 hours after blood ingestion, there was no significant difference in the increasing degree of the activity of the three enzymes in other time after blood ingestion. The activity of the three enzymes also increased during growing up of oocytes.

Key words: Monopsyllus anisus, Leptopsylla segnis, mature process, enzyme, histochemistry