关键词: 灰飞虱；胚胎组织；细胞培养, 培养基

Abstract:

To build cell layer line of Laodelphax striatellus Fallen, seven different full culture media, including MM medium, lactalbumin hydrolysate, yeast extract and fetal bovine serum (FBS) based on Grace medium, were developed and tested to culture the tissues and cells from embryo of L. striatellus. The results indicated that these tissues and cells could survive keeping close to the bottom of medium for at least 1－4 weeks, and for even 2 months in the medium No. 5, which was made up of one Grace medium, one MM medium respectively and 20% FBS. Furthermore, effective hydrolyzation of the tissues was reached when they were pretreated for 5－10 min with 0.25% trypsin under the condition of pH 8.0 at 37℃. The suitable culture recipe to the tissues and cells from embryo of L. striatellus and a good method to separate tissues were proposed.

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Key words: Laodelphax striatellus, embryonic tissues, cell culture, culture media