›› 2005, Vol. 48 ›› Issue (3): 450-454.

• 研究论文 • 上一篇    下一篇

Bt毒素对棉铃虫雄蛾感受雌蛾性信息素EAG反应的影响

穆兰芳,董双林   

  1. 南京农业大学,农业部病虫监测与治理重点开放实验室
  • 出版日期:2005-07-12 发布日期:2005-06-20
  • 通讯作者: 董双林

Effects of Bt toxin on EAG responses of male moths of the cotton bollworm, Helicoverpa armigera(Hübner) to sex pheromone

MU Lan-Fang, DONG Shuang-Lin*   

  1. Nanjing Agricultural University
  • Online:2005-07-12 Published:2005-06-20

摘要:

用含Bt毒素的人工饲料饲养棉铃虫Helicoverpa armigera 3龄幼虫至成虫(死亡率为40%~50%),采用触角电位 (electroantennogram, EAG) 技术,测定了雄蛾对雌蛾性信息素2种组分顺9-十六碳烯醛(Z9-16:Ald)、顺11-十六碳烯醛(Z11-16:Ald)及其混合物(Z11-16:Ald∶Z9-16:Ald=97∶3)的EAG反应。结果表明,Bt毒素对雄蛾感受性信息素单一组分和混合物的EAG反应均具促进作用;且随信息素剂量的增加,这种促进作用也随之增强。这一结果对于评价和实施延缓棉铃虫对Bt棉抗性的“庇护所"策略,具有一定的参考意义。


 

关键词: Bt毒素, 棉铃虫, 性信息素, EAG反应

Abstract:

The thirdinstar larvae of the cotton bollworm, Helicoverpa armigera (Hübner), were raised to adult with artificial diet containing Bt Cry1Ac toxin at 40%-50% lethal dose. Electroantennogram (EAG) responses were recorded for those survival male moths and untreated moths. The results indicated that EAG values of Bt toxin treated males to Z9-16:Ald, Z11-16:Ald and their binary mixture (Z11-16:Ald∶Z9-16:Ald=97∶3) were all higher than those of controls, and that differences of the EAG values between treatments and controls became greater as the concentration of the sex pheromone components increased. These results were meaningful in evaluation and implementation of the “refuge" strategy used to postpone the Bt cotton resistance by the cotton bollworm.


 

Key words: Bt toxin, Helicoverpa armigera, sex pheromone, EAG response