茚虫威亚致死浓度对茚虫威敏感性降低的棉铃虫生物学参数及解毒酶活性的影响

doi:10.16380/j.kcxb.2019.08.008

摘要/Abstract

摘要：

【目的】本研究旨在明确茚虫威亚致死浓度对茚虫威敏感性降低的棉铃虫Helicoverpa armigera生物学参数及解毒酶活性的影响，以科学有效防治这一害虫，避免其对茚虫威的抗性快速发展。【方法】采用浸叶法测定了茚虫威对棉铃虫茚虫威抗性汰选种群(TP)及其同源对照种群(CP)3龄幼虫的毒力；用两性生命表分析LC₂₀浓度茚虫威对TP种群当代(F₀)生命表参数的影响，并测定了LC₂₀浓度茚虫威处理48 h后CP和TP种群棉铃虫3龄幼虫体内解毒酶［多功能氧化酶(MFO)、羧酸酯酶(CarE)和谷胱甘肽-S-转移酶(GST)］及乙酰胆碱酯酶(AChE)的活性。【结果】茚虫威对棉铃虫CP种群和TP种群3龄幼虫的LC₂₀分别为2.27和9.91 mg/L。LC₂₀茚虫威处理TP种群后，48 h的生长量、化蛹率、羽化率和成虫畸形率均显著低于未用药对照，而特定年龄生命期望值e_xj高于未用药对照；TP种群棉铃虫3龄幼虫体内GST和MFO活性与CP种群相比显著升高，CarE活性显著降低。【结论】本研究结果表明棉铃虫TP种群在LC₂₀浓度茚虫威胁迫下存在明显的生长与繁殖不利性，同时对其也产生了适应能力。LC₂₀浓度茚虫威处理后，棉铃虫TP种群的GST和MFO活性被显著诱导，说明这两种酶可能与棉铃虫对茚虫威产生抗药性密切相关；而CarE活性被显著抑制，说明该酶可能参与了茚虫威转化成N-脱甲氧羰基代谢物(DCJW)的活化过程。

关键词: 棉铃虫, 茚虫威, 生物学参数, 亚致死浓度, 酶活性, 诱导效应

Abstract: 【Aim】 This study aims to clarify the effects of sublethal concentration of indoxacarb on the biological parameters and detoxifying enzyme activities in the cotton bollworm, Helicoverpa armigera with reduced susceptibility to indoxacarb, so as to scientifically control this insect pest and to effectively avoid the rapid development of its resistance to indoxacarb. 【Methods】 The toxicities of indoxacarb to the 3rd instar larvae of the indoxacarb-selected population (TP) of H. armigera and its homologous control population (CP) were determined with leaf-dipping method. The effects of indoxacarb at the concentration of LC₂₀on the biological parameters of the TP population of the age-stage (F₀) were analyzed by two-sex life table method, and the activities of detoxification enzymes ［mixed function oxidase (MFO), carboxylesterase (CarE) and glutathione S-transferase (GST)］ and acetylcholinesterase (AchE) in the CP and TP populations after exposure for 48 h were assayed. 【Results】 The LC₂₀values of indoxacarb to the 3rd instar larvae of the CP and TP populations of H. armigera were 2.27 and 9.91 mg/L, respectively. After the 3rd instar larvae of the TP population were exposed to LC₂₀ of indoxacarb for 48 h, their growth, pupation rate, emergence rate and adult malformation rate were significantly lower than those of the untreated control, while their age-stage-specific life expectancy (e_xj) was higher than that of the untreated control. Meanwhile the activities of GST and MFO in the 3rd instar larvae of the TP population were significantly increased while the CarE activity was obviously decreased compared with the CP population. 【Conclusion】 The results showed that TP population of H. armigera shows obvious disadvantages of growth and reproduction under the selection pressure of indoxacarb at the concentration of LC₂₀, and has also developed the adaptability to this concentration of indoxacarb. The induced activities of GSTs and MFO in the TP population by LC₂₀ of indoxacarb indicate that the two enzymes are closely related to the resistance to indoxacarb in H. armigera, while the inhibited CarE activity indicates that CarE may be involved in the activation of indoxacarb converting into N-decarbomethoxyllated indoxacarb (DCJW).

Key words: Helicoverpa armigera; indoxacarb, biological parameters, sublethal concentration, enzyme activity, induction effect

王芹芹, 崔丽, 王奇渊, 王立, 谢娜, 袁会珠, 芮昌辉. 茚虫威亚致死浓度对茚虫威敏感性降低的棉铃虫生物学参数及解毒酶活性的影响[J]. 昆虫学报, 2019, 62(8): 960-969.

WANG Qin-Qin, CUI Li, WANG Qi-Yuan, WANG Li, XIE Na, YUAN Hui-Zhu, RUI Chang-Hui. Effects of sublethal concentration of indoxacarb on the biological parameters and detoxifying enzyme activities in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) with reduced susceptibility to indoxacarb[J]. Acta Entomologica Sinica, 2019, 62(8): 960-969.

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茚虫威亚致死浓度对茚虫威敏感性降低的棉铃虫生物学参数及解毒酶活性的影响

Effects of sublethal concentration of indoxacarb on the biological parameters and detoxifying enzyme activities in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) with reduced susceptibility to indoxacarb

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