›› 2005, Vol. 48 ›› Issue (4): 576-581.

• 研究论文 • 上一篇    下一篇



  1. 浙江大学应用昆虫学研究所
  • 出版日期:2005-09-08 发布日期:2005-08-20
  • 通讯作者: 叶恭银

Identification of early larvae of common carrionbreeding flies by two-dimensional gel electrophoresis finger map

LI Kai, YE Gong-Yin, HU Cui   

  1. Zhejiang University
  • Online:2005-09-08 Published:2005-08-20



关键词: 蛋白质组, 双向凝胶电泳, 法医昆虫学, 尸食性蝇类, 种类鉴别


With the application of two-dimensional gel electrophoresis (2-DE) technology in biology science, the importance of constructing proteome map has been recognized. Protein maps of neonate larvae of four common carrionbreeding fly species were obtained using 2-DE in the range of pH 4-7. After silver stained detection of protein spots, we found that there were marked differences among these maps. Isoelectric points and molecular weight analysis indicated that 2-DE map could be used to identify neonate larvae of common carrion breeding flies which are confused in morphology.




Key words: Proteom, two-dimensional gel electrophoresis, forensic entomology, carrion-breeding flies, species identification