保幼激素类似物对斜纹夜蛾核多角体病毒增殖的影响

摘要/Abstract

摘要：

分别用5×10⁶，1×10⁷，5×10⁷，1×10⁸ PIBs/mL 4种浓度的斜纹夜蛾核多角体病毒(SpltNPV)感染斜纹夜蛾末龄幼虫（第6龄），并于同龄期以10μg/头的保幼激素类似物(JHA) methoprene分别对各感染组进行点滴处理，以研究JHA对SpltNPV增殖的影响。研究表明，与对照组相比各不同浓度处理组病毒总产量分别提高227.06%、128.71%、52.62%、33.15%，平均单头病毒含量分别提高49.15%、48.40%、36.40%和31.11%，病毒感染死亡率分别提高119.21%、52.72%、12.64%和1.12%。其中以1×10.7 PIBs/mL感染再经JHA处理的病毒总产量和平均单头病毒含量最高，分别为1 701.8×10⁸PIBs和60.1×10⁸ PIBs。各处理组的病毒总产量和平均单头病毒含量均显著高于其对照组。在此基础上，进一步研究了JHA对染毒与未染毒宿主消化生理的影响。结果表明，JHA处理不仅延长了6龄幼虫的寿命，增加了其取食量，而且还显著提高了幼虫的食物转化率和病毒产量。

关键词: 斜纹夜蛾, 保幼激素类似物, methoprene, 斜纹夜蛾核多角体病毒, 增殖, 消化生理

Abstract:

The influences of juvenile hormone analogues (JHA) on multiplication of Spodoptera litura nucleopolyhedrovirus were studied. The early 6th instar larvae of Spodoptera litura were infected with four concentrations of SpltNPV, 5×10⁶，1×10⁷，5×10⁷and 1×10⁸ PIBs/mL, respectively, and then treated with 10 μg methoprene (a JHA) per larva. The results indicated that the total virus yield of the four concentration treatments with virus and methoprene increased by 227.06%, 128.71%, 52.62% and 33.15% than that of the control with virus and acetone, the mean yield per larva increased by 49.15%, 48.40%, 36.40% and 31.11% and the mean mortality increased by 119.21%, 52.72%, 12.64% and 1.12%, respectively. The greatest virus yield was obtained when the larvae were infected by 1×10⁷ PIBs/mL and then treated with 10 μg methoprene per larva, and the total virus yield and the mean virus yield per larva were 1 701.8×10⁸ PIBs and 60.1×10⁸ PIBs respectively. The virus yield of every treated group was significantly greater than that of the control group. Furthermore, the effects of methoprene on digestive physiology of infected and non-infected host were also studied. The results showed that not only the life duration and food consumption of 6th instar larvae were increased, the food conversion efficiency and virus yield of the larvae treated with methoprene were also increased significantly.

Key words: Spodoptera litura, juvenile hormone analogue, methoprene, SpltNPV, multiplication, digestive physiology

刘永平, 王方海, 苏志坚, 李广宏, 庞义. 保幼激素类似物对斜纹夜蛾核多角体病毒增殖的影响[J]. , 2005, 48(6): 866-870.

LIU Yong-Ping, WANG Fang-Hai, SU Zhi-Jian, LI Guang-Hong, PANG Yi. Effect of juvenile hormone analogues on multiplication of Spodoptera litura nucleopolyhedrovirus[J]. , 2005, 48(6): 866-870.

[1]	鲁艳辉, 白琪, 郑许松, 田俊策, 吕仲贤. 四种鳞翅目害虫精氨酸激酶基因的表达谱及基于RNAi的功能分析[J]. 昆虫学报, 2019, 62(8): 901-911.
[2]	马康, 邹晓鹏, 岑永杰, 郑思春. Sli-miR-34-5p响应植物次生物质正调控斜纹夜蛾谷胱甘肽S-转移酶基因SlGSTe1的表达[J]. 昆虫学报, 2019, 62(1): 1-8.
[3]	岳文波, 郅军锐, 刘利, 叶茂, 张向前, 曾广 . 害虫取食和机械损伤对菜豆不同部位叶片防御酶活性的影响[J]. , 2018, 61(7): 860-870.
[4]	孙佳斌, 刘乃勇, 李双美, 闫祺, 董双林. 斜纹夜蛾信息素结合蛋白SlitPBP4的分子克隆、组织表达谱及结合特性分析[J]. , 2018, 61(6): 657-667.
[5]	李良德, 王定锋, 刘丰静, 张辉, 李慧玲, 吴光远. 茶尺蠖水通道蛋白EoAQP1的细胞器定位及功能分析[J]. , 2017, 60(7): 750-759.
[6]	梁炜博, 向兴, 王学贵, 杨群芳, 罗家栋. 噻虫啉亚致死剂量对斜纹夜蛾解毒酶系活性与生长繁殖的影响[J]. , 2017, 60(10): 1187-1197.
[7]	范琳芳, 何文婧, 苏智仁, 朱家明, 罗威, 张纯, 黄立华. 斜纹夜蛾酵母双杂交文库的构建及其在蜕皮激素受体互作蛋白筛选中的应用[J]. , 2016, 59(8): 801-811.
[8]	郭成林, 覃柳燕, 万树青, 马跃峰, 覃建林, 马永林. 黄皮种子提取物对斜纹夜蛾幼虫的杀虫活性及有效成分鉴定[J]. , 2016, 59(8): 839-845.
[9]	郝强, 黄倩, 梁炜博, 贡常委, 王学贵. 不同温度下斜纹夜蛾的两性生命表[J]. , 2016, 59(6): 654-662.
[10]	刘佳, 周勇, 朱航, 马海昊, 邓希乐, 周小毛, 柏连阳. 斜纹夜蛾抗药性监测及茚虫威对其解毒代谢酶的影响[J]. , 2016, 59(11): 1254-1262.
[11]	唐维媛, 邢丛丛, 董永刚, 王啸, 张义明. 斜纹夜蛾幼虫感染莱氏野村菌后的抗氧化酶活性变化[J]. , 2015, 58(5): 526-534.
[12]	万新龙, 李江辉, 劳冲, 杜永均. 斜纹夜蛾触角叶神经元及其对植物气味和性信息素的反应[J]. , 2015, 58(3): 223-236.
[13]	林欣大, 劳冲, 姚云, 杜永均. 性信息素对斜纹夜蛾雄蛾嗅觉相关基因 abp, pbp 和 or 表达的影响[J]. , 2015, 58(3): 237-243.
[14]	杨欢欢, 姚丽丽, 张剑韵, 黄龙全. 外源激素处理后家蚕吡哆醛激酶和磷酸吡哆醇氧化酶转录水平的变化[J]. , 2015, 58(12): 1285-1290.
[15]	胡楠, 董战旗, 陈婷婷, 潘敏慧. 家蚕细胞中过表达家蚕核型多角体病毒核衣壳蛋白VP39抑制BmNPV的增殖[J]. , 2015, 58(11): 1222-1228.

保幼激素类似物对斜纹夜蛾核多角体病毒增殖的影响

Effect of juvenile hormone analogues on multiplication of Spodoptera litura nucleopolyhedrovirus

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10