›› 2014, Vol. 57 ›› Issue (1): 67-73.

• 研究论文 • 上一篇    下一篇

南美斑潜蝇幼虫为害对黄瓜叶片中水杨酸和茉莉酸的诱导作用

孙兴华, 周晓榕, 庞保平*, 孟庆玖   

  1. (内蒙古农业大学农学院, 呼和浩特 010019)
  • 出版日期:2014-01-20 发布日期:2014-01-20

Induction of salicylic acid and jasmonic acid in cucumber leaves after infestation by Liriomyza huidobrensis (Diptera: Agromizidae) larvae

SUN Xing-Hua, ZHOU Xiao-Rong, PANG Bao-Ping*, MENG Qing-Jiu   

  1.  (College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China)
  • Online:2014-01-20 Published:2014-01-20

摘要: 【目的】为揭示南美斑潜蝇Liriomyza huidobrensis (Blanchard)与其寄主相互作用的机理, 为利用诱导抗性控制南美斑潜蝇的发生为害奠定必要的基础。【方法】本文采用高效液相色谱法(HPLC)和超高效液相色谱法-质谱联用法(UPLCMS), 分别测定了南美斑潜蝇幼虫为害对黄瓜叶片中茉莉酸(jasmonic acid, JA)和水杨酸(salicylic acid, SA)的诱导作用。【结果】南美斑潜蝇幼虫持续为害1 d后, 受害黄瓜叶片内JA含量即显著高于健康对照, 轻度受害处理和重度受害处理分别在第3天和第5天上升幅度最大, 分别比健康对照增加2.01倍和1.62倍; 而SA含量在3 d后才显著高于健康对照, 轻度受害处理和重度受害处理在第9天上升幅度最大, 分别比健康对照增加4.66倍和1.67倍; 轻度受害对JA和SA的系统诱导作用不明显, 而重度受害对JA和SA具有明显的系统诱导作用。【结论】南美斑潜蝇幼虫为害对黄瓜叶片内JA和SA具有诱导作用。

关键词: 南美斑潜蝇, 幼虫为害, 黄瓜, 诱导抗性, 水杨酸, 茉莉酸

Abstract: 【Aim】 In order to investigate the mechanism of interactions between Liriomyza huidobrensis  (Blanchard) and its host plants and build an indispensable foundation for controlling the fly by use of inducible defenses. 【Methods】 High-performance liquid chromatography (HPLC) and ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) were used to determine the effects of infestation of larval L. huidobrensis on induction of jasmonic acid (JA) and salicylic acid (SA), respectively, in cucumber leaves. 【Results】 The results showed that JA contents in the damaged leaves were significantly higher than those in intact controls only 1 d after the larval continuous infestation, and the increase magnitudes in the light-damage treatment and the severedamage treatment reached the maximum at 3-5 d after larval infestation, increasing 2.01 and 1.62 times as compared with the intact control, respectively. SA contents were significantly higher at 3 d after larval infestation, and the increase magnitudes in the lightdamage treatment and the severe-damage treatment reached the maximum at 9 d after larval infestation, rising 4.66 and 1.67 times as compared with the intact control, respectively. The induction of JA and SA was significantly systemic in the severe-damage treatment but not in the lightdamage treatment. 【Conclusion】 The results suggest that the larval infestation of L. huidobrensis can induce the JA and SA increase in cucumber leaves.

Key words: Liriomyza huidobrensis, larval infestation, cucumber, inducible defense, jasmonic acid (JA), salicylic acid (SA)