感染和未感染南方水稻黑条矮缩病毒的白背飞虱成虫唾液腺转录组比较分析

doi:10.16380/j.kcxb.2018.04.007

摘要/Abstract

摘要： 【目的】白背飞虱Sogatella furcifera是南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus, SRBSDV）传播的主要介体，其唾液腺在取食和传毒的过程中都发挥着重要作用，本研究旨在通过对带毒与不带毒白背飞虱成虫的唾液腺进行转录组测序，进而比较分析找出差异表达基因，推测出唾液腺中与传毒相关的基因。【方法】利用Ion ProtonⅡ, PGM平台进行带毒与不带毒白背飞虱成虫唾液腺转录组测序，用SOAPdenovo软件从头组装，通过blastX比对进行基因注释。采用Blast2go和Blastall软件对所测基因的GO功能富集和KEGG代谢途径进行分析；釆用RPKM分析差异表达基因。【结果】带毒和不带毒白背飞虱的唾液腺转录组样本的原始数据经过软件处理后分别获得52 062和51 407条unigenes序列（GenBank登录号分别为SRS851833和SRS843978），平均长度分别为639和647 bp。通过NR数据库比对，共18 431个unigenes具有同源序列，与赤拟谷盗Tribolium castaneum的同源序列最多（16.23%）。所有unigenes富集到52个GO类群，240个KEGG信号通路。白背飞虱在感染南方水稻黑条矮缩病毒后，唾液腺有89个unigenes序列的表达发生变化。【结论】本研究通过比较分析带毒与不带毒白背飞虱唾液腺转录组样本后，发现有部分基因序列的表达存在差异，这些基因序列可能和病毒与载体的互作有关。转录组数据的获得为研究与取食和传毒相关的基因提供了有用的信息，从而在分子水平上为后续研究白背飞虱与SRBSDV互作机制奠定基础。

关键词: 白背飞虱, 唾液腺, 南方水稻黑条矮缩病毒, 转录组, 差异表达基因

Abstract: 【Aim】 The white-backed planthopper (WBPH), Sogatella furcifera, is a main vector of Southern rice black-streaked dwarf virus (SRBSDV). The salivary glands of the WBPH play an important role in feeding behaviour and virus transmission. This study aims to sequence the salivary gland transcriptomes of viruliferous (SRBSDV-infected) and nonviruliferous adults of the WBPH, to find the differentially expressed genes in salivary glands and to further infer the virus-related genes. 【Methods】 Salivary gland transcriptomes of viruliferous and nonviruliferous adults of S. furcifera were sequenced by using the Ion Proton II, PGM platform, and then were de novo assembled by SOAPdenovo software. Gene annotation was conducted by blastX, and GO term enrichment and KEGG metabolic pathway analysis were performed using Blast2go and Blastall software. The differentially expressed genes were calculated by RPKM values. 【Results】 A total of 52 062 unigenes from viruliferous salivary glands (VSGs) and 51 407 unigenes from nonviruliferous salivary glands (NVSGs) of S. furcifera with the mean length of 639 and 647 bp, respectively, were obtained, and their GenBank accession numbers are SRS851833 and SRS843978, respectively. A total of 18 431 unigenes have homologous sequences against the NR database, and the highest percentage of unigene sequences (16.23%) were matched to genes of Tribolium castaneum. All unigenes were enriched to 52 GO terms and 240 KEGG pathways. The results revealed that 89 unigenes had a significantly different expression level between VSGs and NVSGs. 【Conclusion】 Through the comparative analysis, the differentially expressed genes between viruliferous and nonviruliferous salivary glands of S. furcifera were found, and some of these genes might be involved in virus-vector interactions. The gene expression characteristics of salivary gland transcriptome provide useful information for the identification of genes involved in feeding and virus transmission and so lay the basis for investigating the interaction between SRBSDV and WBPH at the molecular level.

Key words: Sogatella furcifera; salivary glands, Southern rice black-streaked dwarf virus (SRBSDV), transcriptome, differentially expressed genes

邓瑶, 刘玉娣, 王香萍, 侯茂林. 感染和未感染南方水稻黑条矮缩病毒的白背飞虱成虫唾液腺转录组比较分析[J]. 昆虫学报, 2018, 61(4): 449-457.

DENG Yao, LIU Yu-Di, WANG Xiang-Ping, Hou-Mao-Lin. Comparative transcriptome analysis of salivary glands of Southern rice black-streaked dwarf virus (SRBSDV)-infected and uninfected adults of the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)[J]. Acta Entomologica Sinica, 2018, 61(4): 449-457.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.insect.org.cn/CN/10.16380/j.kcxb.2018.04.007

http://www.insect.org.cn/CN/Y2018/V61/I4/449

[1]	张博, 韩海斌, 徐林波, 高书晶, 甘霖, 岳方正, 刘爱萍. 伞裙追寄蝇滞育关联基因的转录组学分析[J]. 昆虫学报, 2020, 63(8): 932-940.
[2]	姚丹, 周姝婧, 周冰峰, 徐新建, 朱翔杰. 低温胁迫下意大利蜜蜂预蛹的差异表达基因趋势分析[J]. 昆虫学报, 2020, 63(6): 688-696.
[3]	杨雪娇, 张蔓, 江宇航, 唐启河, 张棋麟, 林连兵, 陈均远. 七星瓢虫触角转录组及嗅觉相关基因分析[J]. 昆虫学报, 2020, 63(6): 717-726.
[4]	廖永林, 李燕芳, 杨斌, 赵华, 高燕, 陈伟平, 蔡时可, 王继华. 草地贪夜蛾雄性成虫和5龄幼虫的转录组比较分析[J]. 昆虫学报, 2020, 63(5): 523-532.
[5]	孙丽娜, 张怀江, 刘孝贺, 仇贵生. 基于转录组的苹小卷叶蛾杀虫剂靶标及解毒代谢相关基因分析[J]. 昆虫学报, 2020, 63(4): 470-481.
[6]	耿四海, 周丁丁, 范小雪, 蒋海宾, 祝智威, 王杰, 范元婵, 王心蕊, 熊翠玲, 郑燕珍, 付中民, 陈大福, 郭睿. 转录组分析揭示东方蜜蜂微孢子虫侵染意大利蜜蜂的分子机制[J]. 昆虫学报, 2020, 63(3): 294-308.
[7]	李鸣宇, 周娇, 王海香, 赵莉蔺. 携带与未携带松材线虫的松墨天牛转录组差异表达基因分析[J]. 昆虫学报, 2020, 63(2): 207-217.
[8]	贾路瑶, 刘丹丹, 侯茂林. 水稻施硅对白背飞虱刺吸和寄主选择行为的影响[J]. 昆虫学报, 2020, 63(2): 199-206.
[9]	陈华枝, 杜宇, 范小雪, 祝智威, 蒋海宾, 王杰, 范元婵, 熊翠玲, 郑燕珍, 付中民, 徐国钧, 陈大福, 郭睿. 基于第三代纳米孔测序技术的东方蜜蜂微孢子虫全长转录组构建及注释[J]. 昆虫学报, 2020, 63(12): 1461-1472.
[10]	杜宇, 付中民, 祝智威, 王杰, 冯睿蓉, 王秀娜, 蒋海宾, 范元婵, 范小雪, 熊翠玲, 郑燕珍, 徐国钧, 陈大福, 郭睿. 基于蜜蜂球囊菌纳米孔测序数据的基因非翻译区延长、SSR位点发掘及未注释基因和转录本鉴定[J]. 昆虫学报, 2020, 63(11): 1345-1357.
[11]	刘杰, 李勃, 陈晓洁, 陈斌. 埃及伊蚊不同组织的基因共表达模式分析[J]. 昆虫学报, 2020, 63(10): 1171-1182.
[12]	巩雪芳, 谢寿安, 杨平, 郭莉, 陈迪, 车显荣, 王延来, 吕淑杰. 花椒窄吉丁触角转录组及嗅觉相关基因分析[J]. 昆虫学报, 2020, 63(10): 1159-1170.
[13]	高成龙, 敖特根, 任利利, 王立祥, 王明, 石娟. 松树蜂毒腺转录组分析[J]. 昆虫学报, 2019, 62(9): 1038-1047.
[14]	王超, 包花尔, 乌云高娃, 崔阿拉腾乌拉, 巴音吉日嘎拉, 额尔敦木图. 黑须污蝇不同发育阶段转录组及嗅觉相关基因分析[J]. 昆虫学报, 2019, 62(6): 685-693.
[15]	黎东海, 赵萍. 基于转录组数据的齿缘刺猎蝽微卫星分子标记开发[J]. 昆虫学报, 2019, 62(6): 694-702.

感染和未感染南方水稻黑条矮缩病毒的白背飞虱成虫唾液腺转录组比较分析

Comparative transcriptome analysis of salivary glands of Southern rice black-streaked dwarf virus (SRBSDV)-infected and uninfected adults of the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10