陕西关中地区禾谷缢管蚜田间种群对拟除虫菊酯杀虫剂的抗性监测及钠通道基因M918L突变检测

doi:10.16380/j.kcxb.2025.10.004

昆虫学报 ›› 2025, Vol. 68 ›› Issue (10): 1339-1350.doi: 10.16380/j.kcxb.2025.10.004

陕西关中地区禾谷缢管蚜田间种群对拟除虫菊酯杀虫剂的抗性监测及钠通道基因M918L突变检测

张思茜, 张愉英, 高平, 周玉荣, 彭雄, 王康^*, 陈茂华^*

(西北农林科技大学植物保护学院, 农业部西北黄土高原作物有害生物综合治理重点实验室, 作物抗逆与高效生产全国重点实验室, 杨凌 712100)

出版日期:2025-10-20 发布日期:2025-11-28

Resistance monitoring of the field populations of Rhopalosiphum padi (Hemiptera: Aphididae) from the Guanzhong Region of Shaanxi Province, nothwestern China to pyrethroid insecticides and detection of the M918L mutation in the voltage-gated sodium channel gene

ZHANG Si-Qian, ZHANG Yu-Ying, GAO Ping, ZHOU Yu-Rong, PENG Xiong, WANG Kang^*, CHEN Mao-Hua^*

(Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, State Key Laboratory for Crop Stress Resistance and HighEfficiency Production, College of Plant Protection, Northwest A&F University, Yangling 712100, China)

Online:2025-10-20 Published:2025-11-28

摘要/Abstract

摘要： 【目的】明确陕西关中地区禾谷缢管蚜Rhopalosiphum padi田间种群对5种拟除虫菊酯杀虫剂的抗性水平以及拟除虫菊酯靶标基因电压门控钠离子通道（voltage-gated sodium channel, VGSC）基因突变频率与抗药性水平间的相关性。【方法】2023和2024年4-6月采集陕西关中地区9个禾谷缢管蚜田间种群，采用带虫浸叶法测定无翅成蚜对联苯菊酯、氯菊酯、高效氯氟氰菊酯、溴氰菊酯和氰戊菊酯5种拟除虫菊酯类杀虫剂的敏感性； PCR检测各禾谷缢管蚜田间种群VGSC基因突变类型及频率，分析突变频率与抗性水平(抗性倍数)间的相关性。【结果】生物测定结果表明，各禾谷缢管蚜田间种群无翅成蚜对5种拟除虫菊酯类杀虫剂已产生不同程度的抗性。2023年采自咸阳市兴平县的种群(XP-2023种群)无翅成蚜对Ⅰ型拟除虫菊酯联苯菊酯和氯菊酯的抗性水平最高，抗性倍数分别为76.1和52.3； 2024和2023年采自宝鸡市扶风县的种群(FF-2024和FF-2023种群)及2023年采自咸阳市礼泉县的种群(LQ-2023种群)无翅成蚜分别对Ⅱ型拟除虫菊酯高效氯氟氰菊酯、溴氰菊酯和氰戊菊酯的抗性水平最高，抗性倍数分别为60.7, 97.5和42.6。VGSC基因突变检测发现，除2023年采自咸阳市武功县和宝鸡市岐山县的种群(WG-2023和QS-2023种群)外，其他禾谷缢管蚜田间种群VGSC基因均存在M918L杂合突变，突变频率在5.0%~47.5%之间， M918L的突变频率与对拟除虫菊酯类杀虫剂的抗性水平存在显著的相关性。【结论】 VGSC基因M918L杂合突变与禾谷缢管蚜田间种群对拟除虫菊酯类杀虫剂抗性水平存在显著的相关性，这一靶标突变在禾谷缢管蚜田间种群对拟除虫菊酯的抗性中具有重要作用。田间防治禾谷缢管蚜建议少用或不用拟除虫菊酯类杀虫剂，同时需要持续监测田间种群的抗性水平。

关键词: 禾谷缢管蚜, 拟除虫菊酯, 抗药性监测, 靶标突变, 电压门控钠离子通道, 基因突变

Abstract: 【Aim】 To ascertain the resistance levels of the field populations of Rhopalosiphum padi from the Guanzhong Region of Shaanxi Province, nothwestern China to five pyrethroid insecticides and analyze the correlations between the mutation frequencies of the voltage-gated sodium channel (VGSC) gene (the target gene of pyrethroid) of various field populations and resistance levels. 【Methods】Nine field populations of R. padi were collected from the Guanzhong Region of Shaanxi Province during April-June in 2023 and 2024 and the susceptibility of the apterous adults to five pyrethroid insecticides (bifenthrin, permethrin, lambda-cyhalothrin, deltamethyrin and fenvalerate) was tested by leaf-dipping method. PCR was used to detect the types and frequencies of VGSC gene mutation of various field populations of R. padi. The correlation between the mutation frequency and the resistance level (resistance ratio, RR) was analyzed. 【Results】Bioassay results revealed that the apterous adults of various field populations of R. padi exhibited varying degrees of resistance to five pyrethroid insecticides. The apterous adults of the population collected from Xingping County, Xianyang City in 2023 (XP-2023) showed the highest resistance levels to type Ⅰ pyrethroid insecticides (bifenthrin and permethrin), with the RRs of 76.1 and 52.3, respectively. The apterous adults of the populations collected from Fufeng County, Baoji City in 2024 and 2023 (FF-2024 and FF-2023) and the population collected from Liquan County, Baoji City in 2023 (LQ-2023) showed the highest resistance levels to type Ⅱ pyrethroid insecticides (lambda-cyhalothrin, deltamethrin and fenvalerate), with the RRs of 60.7, 97.5 and 42.6, respectively. Detection of VGSC gene mutations revealed that, except for the populations collected from Wugong County, Xianyang City and Qishan County, Baoji City, respectively, in 2023 (WG-2023 and QS-2023), all the field populations carried heterozygous mutation (M918L) in the VGSC gene, with the mutation frequencies ranging from 5.0% to 47.5%. The mutation frequency of M918L was significantly correlated with the level of resistance to pyrethroid insecticides. 【Conclusion】The M918L heterozygous mutation in the VGSC gene is significantly correlated with the resistance levels of the field populations of R. padi to pyrethroid insecticides, indicating that this target mutation plays an important role in the resistance of the field populations of R. padi to these insecticides. It is recommended to minimize or avoid the use of pyrethroid insecticides in field control of R. padi and to continuously monitor the resistance levels of field populations.

Key words: Rhopalosiphum padi, pyrethroid, resistance monitoring, target mutation, voltage-gated sodium channel, gene mutation

张思茜, 张愉英, 高平, 周玉荣, 彭雄, 王康, 陈茂华. 陕西关中地区禾谷缢管蚜田间种群对拟除虫菊酯杀虫剂的抗性监测及钠通道基因M918L突变检测[J]. 昆虫学报, 2025, 68(10): 1339-1350.

ZHANG Si-Qian, ZHANG Yu-Ying, GAO Ping, ZHOU Yu-Rong, PENG Xiong, WANG Kang, CHEN Mao-Hua. Resistance monitoring of the field populations of Rhopalosiphum padi (Hemiptera: Aphididae) from the Guanzhong Region of Shaanxi Province, nothwestern China to pyrethroid insecticides and detection of the M918L mutation in the voltage-gated sodium channel gene[J]. Acta Entomologica Sinica, 2025, 68(10): 1339-1350.

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陕西关中地区禾谷缢管蚜田间种群对拟除虫菊酯杀虫剂的抗性监测及钠通道基因M918L突变检测

Resistance monitoring of the field populations of Rhopalosiphum padi (Hemiptera: Aphididae) from the Guanzhong Region of Shaanxi Province, nothwestern China to pyrethroid insecticides and detection of the M918L mutation in the voltage-gated sodium channel gene

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