昆虫学报 ›› 2016, Vol. 59 ›› Issue (7): 707-715.doi: 10.16380/j.kcxb.2016.07.002

• 研究论文 • 上一篇    下一篇

西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响

郅军锐1,*, 田甜1,2,3, 温娟1, 刘勇1   

  1. (1. 贵州大学昆虫研究所, 贵州省山地农业病虫害重点实验室, 贵阳 550025; 2. 南京农业大学植物保护学院, 南京 210095; 3. 江苏省农业科学院植物保护研究所, 南京 210014)
  • 出版日期:2016-07-20 发布日期:2016-07-20

Effects of kidney bean damaged by Frankliniella occidentalis (Thysanoptera: Thripidae) or Tetranychus urticae (Acari: Tetranychidae) on the activities of protective and detoxification enzymes in the other subsequent herbivore of both

ZHI Jun-Rui1,*, TIAN  Tian1,2,3, WEN  Juan1, LIU  Yong1   

  1. (1. Institute of Entomology, Guizhou University, Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang, 550025, China; 2. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 3. Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing 210014, China)
  • Online:2016-07-20 Published:2016-07-20

摘要: 【目的】害虫取食后会导致植物的防御反应;取食同种植物的不同种害虫其生理适应性可能不同。本研究旨在阐明一种害虫取食后的植物对后取食的另一种害虫虫体酶活性的影响。【方法】采用生化分析法研究了西花蓟马Frankliniella occidentalis 2龄若虫或二斑叶螨Tetranychus urticae雌成螨为害后的菜豆对后取食不同时间下的二斑叶螨第2若螨和雌成螨及西花蓟马2龄若虫和雌成虫体内保护酶[过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)]和解毒酶[多功能氧化酶(MFO)、羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和乙酰胆碱酯酶(AchE)]活性的影响。【结果】二斑叶螨雌成螨取食西花蓟马为害菜豆5 h时,POD, CAT和SOD这3种保护酶的活性均明显高于取食健康植株(P<0.01),取食蓟马为害的菜豆18 h时只有POD活性低于取食健康植株(P<0.01);二斑叶螨第2若螨取食蓟马为害菜豆后只有CAT活性在5 h时明显高于取食健康植株(P<0.01)。二斑叶螨雌成螨取食蓟马为害菜豆5 h时,解毒酶MFO和CarE的活性受到抑制(P<0.01),但GSTs和AchE活性均比取食健康菜豆高(P<0.01);取食18 h时,只有MFO 的活性变化与5 h不同。第2若螨取食蓟马为害菜豆只有MFO 的活性不论在5 h 还是18 h 时明显高于取食健康菜豆(P<0.01)。西花蓟马取食二斑叶螨雌成螨为害菜豆时,雌成虫体内的3种保护酶的活性不论取食5 h 还是18 h均明显升高(P<0.01),但2龄若虫只有CAT活性在这两个时间段下明显升高(P<0.01)。西花蓟马雌成虫取食螨害菜豆5 h时,解毒酶活性的变化同二斑叶螨雌成螨,取食18 h 时只有GSTs活性变化状态与取食5 h不同;西花蓟马2龄若虫取食螨害菜豆时除GSTs外解毒酶的活性变化在这两个时间下正好相反。【结论】西花蓟马或二斑叶螨为害的菜豆能诱导后取食的二斑叶螨和西花蓟马产生一系列应激生化反应,且两种害虫成虫体内所有的保护酶和解毒酶活性在5 h 时变化状态相同,但在其余时间和虫态下酶活性的变化状态不同。说明这两种昆虫体内保护酶和解毒酶活性的变化既有共性,又与害虫种类、虫(螨)态、取食时间相关,两种害虫对虫害植物的生理适应性不完全相同。

关键词: 关键词: 二斑叶螨, 西花蓟马, 菜豆, 保护酶, 解毒酶, 酶活性, 生理适应性

Abstract: 【Aim】 Insect feeding can induce the defense responses of host plants. Different species feeding on the same host plant may have different physiological adaptation. This study aims to investigate the effects of plant damaged by one pest on the enzyme activities in the other subsequent herbivore. 【Methods】 Biochemistry analysis method was used to study the changes in activities of protective enzymes [peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD)] and detoxification enzymes [mixed-function oxidase (MFO), carboxylesterase (CarE), glutathione S-transferase (GSTs) and acetylcholin esterase (AchE)] in deutonymphs and female adults of Tetranychus urticae at different time points after feeding on kidney bean plants damaged by the 2nd instar nymphs of Frankliniella occidentalis and in the 2nd instar nymphs and female adults of F. occidentalis at different time points after feeding on bean plants damaged by female adults of T. urticae. 【Results】 The activities of POD, CAT and SOD in female adults of T. urticae at 5 h after feeding on bean plants damaged by F. occidentalis (BPDF) were significantly higher than those after feeding on healthy bean plants (HBP) (P<0.01), whereas the POD activity in female adults at 18 h after feeding on BPDF was lower than that after feeding on HBP (P<0.01). The CAT activity in deutonymphs of T. urticae at 5 h after feeding on BPDF was significantly higher than that after feeding on HBP (P<0.01). At 5 h after feeding on BPDF, the activities of MFO and CarE in female adults of T. urticae were suppressed (P<0.01), while the GSTs and AchE activities were higher than those after feeding on HBP (P<0.01). At 18 h after feeding on BPDF, only MFO activity was different from that at 5 h. Only MFO activities in deutonymphs of T. urticae at 5 h or 18 h after feeding on BPDF were higher than those after feeding on HBP (P<0.01). The activities of POD, CAT, and SOD in female adults of F. occidentalis at 5 h or 18 h after feeding on bean plants damaged by T. urticae (BPDT) significantly increased (P<0.01), whereas only the CAT activity in 2nd instar nymphs significantly rose as compared with the control (P<0.01). At 5 h after feeding on BPDT, the changes in detoxification enzymes activities in female adults of F. occidentalis were the same as those in female adults of T. urticae, while at 18 h after feeding, their GSTs activities were different from those at 5 h after feeding. Changes in the activities of detoxification enzymes except GSTs in the 2nd instar nymphs of F. occidentalis were just the opposite at 5 h and at 18 h after feeding. 【Conclusion】 Bean plants damaged by F. occidentalis or T. urticae could cause a series of stress responses in the other subsequent herbivore between T. urticae and F. occidentalis. Changes in activities of protective and detoxification enzymes in adults of the two pests at 5 h after feeding were the same, while they were different at other time after feeding and in other developmental stages. The results suggest that the activities of protective and detoxification enzymes in the two pests have somewhat similar change patterns and their changes also are related with pest species, developmental stages and feeding time. The two pests might have various mechanisms of physiological adaptation to plants damaged by insect herbivores.

Key words: Key words: Tetranychus urticae, Frankliniella occidentalis, kidney bean, protective enzymes, detoxification enzymes, enzyme activity, physiological adaptation