›› 2018, Vol. 61 ›› Issue (9): 1003-1009.doi: 10.16380/j.kcxb.2018.09.001

• 研究论文 •    下一篇

温度胁迫对点蜂缘蝽成虫呼吸代谢关键酶活性的影响

陈菊红1,2, 崔娟1, 张金平2, 毕锐1, 高宇1, 徐伟1, 史树森1,*   

  1.  (1. 吉林农业大学农学院, 长春 130118; 2. 中国农业科学院植物保护研究所, 农业部-CABI生物安全联合实验室, 北京 100193)
  • 出版日期:2018-09-20 发布日期:2018-09-20

Effects of temperature on the activities of key enzymes related to respiratory metabolism in Riptortus pedestris (Hemiptera: Coreidae) adults

CHEN Ju-Hong1,2, CUI Juan1, ZHANG Jin-Ping2, BI Rui1, GAO Yu1, XU Wei1, SHI Shu-Sen1,*   

  1. (1. College of Agriculture, Jilin Agricultural University, Changchun 130118, China; 2. MoA-CABI Joint Laboratory for Bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Online:2018-09-20 Published:2018-09-20

摘要: 【目的】为明确温度对点蜂缘蝽Riptortus pedestris成虫呼吸代谢关键酶活性的影响。【方法】本研究利用生化方法测定了黑暗条件下经16, 20, 24, 28, 32和36℃处理4 h的点蜂缘蝽成虫呼吸代谢关键酶3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)、3-羟酰辅酶A脱氢酶(HOAD)、柠檬酸合成酶(CS)和乳酸脱氢酶(LDH)的活性。【结果】点蜂缘蝽成虫体内5种呼吸代谢关键酶的活性均随温度升高呈现先增后减的趋势。其中点蜂缘蝽成虫雌、雄个体之间的CS活性在16和28℃时存在显著差异,16℃时雌虫CS活性较高,28℃时雄虫CS活性较高;雄虫LDH活性在36℃时显著高于雌虫,提示雌、雄个体间在低温下体内三羧酸循环代谢存在显著差异,在高温下体内糖无氧酵解水平存在显著差异。不同温度处理下雌、雄成虫的GAPDH与HOAD活性比值均远大于1.0,说明在试验温度下点蜂缘蝽呼吸代谢消耗以糖类为主。【结论】在16℃~36℃范围内,随着温度的升高点蜂缘蝽雌、雄成虫体内呼吸代谢关键酶活性呈现先增后减的趋势,点蜂缘蝽可通过调节呼吸代谢强度适应温度变化。

关键词: 点蜂缘蝽, 温度, 呼吸代谢, 酶活性, 能源物质

Abstract: 【Aim】 This study aims to determine the effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of the bean bug, Riptortus pedestris. 【Methods】 The activities of five key enzymes related to respiratory metabolism, i.e., glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate (GDH), 3-hydroxyacyl-CoA dehydrogenase (HOAD), citrate synthase (CS), and lactate dehydrogenase (LDH), in R. pedestris adults exposed to different temperatures (16, 20, 24, 28, 32 and 36℃) for 4 h in scotophase were assayed by using biochemical methods. 【Results】 The results showed that the activities of the five key enzymes in R.pedestris adults increased first and then decreased as the temperature increased. The CS activities at 16 and 28℃ differed significantly between males and females, being higher in females than in males at 16℃, but lower in females than in males at 28℃. The LDH activities in males were higher than those in females at 36℃, suggesting that there are significant differences between males and females in the tricarboxylic acid cycle metabolism at low temperature and in the anaerobic glycolysis at high temperature. The ratios of GAPDH activity to HOAD activity in adult males and females exposed to different temperatures were much higher than 1.0, indicating that R. pedestri adults mainly consume carbohydrates in respiratory metabolism at the test temperatures. 【Conclusion】 In the temperature range from 16℃ to 36℃, with the increase of temperature, the activities of key enzymes involved in respiratory metabolism in female and male adults of R. pedestris increase at first, and then decrease. R. pedestris can adjust its respiratory metabolic intensity to some extent to adapt to temperature change.

Key words: Riptortus pedestris, temperature, respiratory metabolism, enzyme activity, energy substance