Abstract:

 【Aim】 This study aims to investigate the infection of secondary endosymbiont Wolbachia in Aphis gossypii populations from different areas of Henan province, central China, and further to ascertain its infection type and phylogeny. 【Methods】 A. gossypii samples were collected from 13 areas ofHenanprovince during 2016-2017, and identified by amplifying the COI gene. The infection rate of Wolbachia was detected by amplification of the wsp gene sequence from the Wolbachia in different A. gossypii populations. The phylogenetic relationship of Wolbachia in different A. gossypii populations was constructed with neighbor-joining method. 【Results】 The infection rates of Wolbachia in 13 populations of A. gossypii collected from different areas ofHenanprovince were different, being the highest (46.67%) in Zhengzhou (ZZ) population and the lowest (6.67%) in Xinyang 2 (XY2) population. The infection rates of Wolbachia in the 13 populations ranged from 6.67% to 46.67%, with the average value of 28.35%. The phylogenetic relationship based on wsp gene indicated that Wolbachia strains in both Anyang and Xinyang populations of A. gossypii were identified as supergroup B, while those in other populations were supergroup A. 【Conclusion】 The infection rate of Wolbachia in different A. gossypii populations of Henan province varies distinctly, and either supergroup A or B Wolbachia is present in these A. gossypii populations.

Key words: Aphis gossypii, Wolbachia, wsp gene, phylogenetic analysis, infection rate, strain