Acta Entomologica Sinica ›› 2019, Vol. 62 ›› Issue (9): 1038-1047.doi: 10.16380/j.kcxb.2019.09.004

• RESEARCH PAPERS • Previous Articles     Next Articles

Analysis of the venom gland transcriptome of Sirex noctilio (Hymenoptera: Siricidae)

GAO Cheng-Long1, AO Te-Gen2, REN Li-Li1, WANG Li-Xiang1, WANG Ming1, SHI Juan1,*   

  1. (1. Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China; 2. Tongliao Control and Quarantine Station of Forest Pest, Tongliao, Inner Mongolia 028000, China)
  • Online:2019-09-20 Published:2019-09-03

Abstract: 【Aim】 To establish the venom gland transcriptome database of the wood wasp, Sirex noctilio, and to screen and analyze the genetic data of its venom gland. 【Methods】 The venom gland transcriptome of S. noctilio female adults was sequenced using an Illumina HiSeqTM 4000 platform, assembled and subjected to bioinformatics analysis. 【Results】 A total of 12.7 Gb clean data of the venom gland transcriptome of S. noctilio female adults was obtained, and the clean reads were assembled into 37 098 unigenes with a mean length of 968 bp and an N50 length of 2 364 bp. A total of 13 515 unigenes were annotated using BlastX searches against the databases. Most unigenes (11 108 unigenes) were annotated to the NR database (accounting for 29.94% of the total), showing the highest homology with the unigenes of Nasonia vitripennis in the NR database by 815 similar unigenes (accounting for 7.29% of the total). A total of 5 726 unigenes were annotated in the GO database and divided into three categories (biological processes, cellular component and molecular function) and 63 subclasses based on their biological functions. In KEGG database, 7 602 unigenes were assigned to the 357 known metabolic pathways. By further screening and identification, 43 olfaction-related genes of S. noctilio including 25 odorant receptor (OR) genes, 10 chemosensory protein (CSP) genes, five ionotropic receptor (IR) genes, and three odorant binding protein (OBP) genes were obtained. In addition, 11 laccase genes including five laccase-1 genes, four laccase-2 genes, one laccase-4 gene and one laccase-9 gene were screened. Among the annotated genes, a LAC2 gene had the highest expression level, with the FPKM value of 21 126. 【Conclusion】 The venom gland transcriptome data of S. noctilio obtained in this study provide a theoretical foundation for the component identification and biological function study of S. noctilio venom.

Key words: Sirex noctilio; venom gland, transctiptome, database, olfactionrelated gene, laccase