Acta Entomologica Sinica ›› 2020, Vol. 63 ›› Issue (4): 470-481.doi: 10.16380/j.kcxb.2020.04.010

• RESEARCH PAPERS • Previous Articles     Next Articles

Transcriptomic analysis of insecticide target and detoxification related genes in Adoxophyes orana (Lepidoptera: Tortricidae)

SUN Li-Na, ZHANG Huai-Jiang, LIU Xiao-He, QIU Gui-Sheng*   

  1.  (Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China)
  • Online:2020-04-20 Published:2020-05-08

Abstract:  【Aim】 To establish the transcriptome database of Adoxophyes orana, and to explore insecticide target and detoxification related genes. 【Methods】 The transcriptome of A. orana was sequenced by Illumina HiSeqTM 2000 sequencing platform to explore insecticide target genes. The expression levels of six insecticide target genes from A. ornan at different developmental stages including egg, larva, pupa and adult were identified and verified using qPCR. The metabolic pathway and evolutionary relationship of 
detoxification related genes in the transcriptome of A. orana were analyzed. 【Results】 A total of 48 610 unigenes (GenBank accession no.: GGMW00000000) were identified by assembling valid sequences. One hundred and fifty-five insecticides target genes were identified. The qPCR results confirmed that there was difference in the expression levels of six insecticide target genes including one ecdysone receptor (ECR) gene, two acetylcholinesterase (AChE) genes, one chloride channel (CLC) gene, one chitinase (CS) gene 
and one ryanodine receptor (RyR) gene at different developmental stages of A. ornanAdditionally, 69 carboxylesterase (CarE) unigenes, 66 glutathion S-transferase (GST) unigenes and 205 cytochrome P450 unigenes were identified. A total of 20 CarE, 32 GST and 30 P450 unigenes were classified into the metabolism categories related to toxic substance metabolism. The results of cluster analyses based on the amino acid sequences of unigenes with complete ORF showed that 9 of 12 CarEs were classified to G class (Lepidoptera neotenin), 10 AoGSTs into subfamily Delta and another 10 AoGSTs into subfamily Epsilon, and all of 18 P450s into CYP3 group. 【Conclusion】 This study contributes to the discovery of insecticide target genes and the research of pesticide resistance in A. orana.

Key words: Adoxophyes orana, transcriptome, insecticide target gene, detoxification related gene, insecticide resistance