Acta Entomologica Sinica ›› 2020, Vol. 63 ›› Issue (9): 1081-1090.doi: 10.16380/j.kcxb.2020.09.005

• RESEARCH PAPERS • Previous Articles     Next Articles

Effects of chlorogenic acid on the growth and development and detoxification-related protein activities in Hyphantria cunea(Lepidoptera: Arctiidae) larvae

PAN Zhong-Yu, MO Xia-Na, MENG Xiang, CHEN Min*   

  1.  (Key Laboratory of Beijing for Forest Pest Control, Beijing Forestry University, Beijing 100083, China)
  • Online:2020-09-20 Published:2020-09-30

Abstract: 【Aim】 This study aims to unveil the effects of chlorogenic acid on the growth and development and detoxification-related protein activities in the larvae of the fall webworm, Hyphantria cunea. 【Methods】 H. cunea larvae were fed with the artificial diets containing different concentrations of chlorogenic acid (0, 0.125%, 0.250%, 0.500%, 1.000% and 2.000%), and the mortality rates of the 5th instar larvae in various groups within 6 d, the nutritional efficiency parameters of the 5th instar larvae in various groups at 48 h after feeding, the growth and development of the larvae (from the 3rd instar) in the 0.500% chlorogenic acid treated group, and the detoxification-related protein activities in the gut of the 5th instar larvae in various groups at 36 h after feeding were determined. 【Results】 The mortality rates of the 5th instar larvae of H. cunea in the chlorogenic acid treated groups in 6 d increased with the increase of the concentration of chlorogenic acid in the artificial diets, and were significantly higher than that in the control group (fed on the diet with 10% DMSO). Chlorogenic acid had significant influences on the nutritional efficiency of the 5th instar larvae of H. cunea. The relative consumption rate (RCR) and approximate digestibility (AD) in the chlorogenic acid treated groups were significantly higher than those in the control group, while the efficiency of conversation of digested food (ECD) in the chlorogenic acid treated groups, and the efficiency of conversation of ingested food (ECI) and relative growth rate (RGR) in the chlorogenic acid treated groups except the 0.125% chlorogenic acid treated group were significantly lower than those in the control. The 0.500% chlorogenic acid extended the duration of the 3rd-5th instar larvae by 28.24% (3.7 d) while shortened the duration of the mature larvae (6th-7th instar) by 8.97% (0.7 d). Moreover, the developmental parameters such as the larval survival rate, pupation rate, eclosion rate, female-to-male sex ratio and oviposition amount in the 0.500% chlorogenic acid treated group were significantly lower than those in the control group. Various concentrations of chlorogenic acid had significant influences on the activities of CYP450, GSTs, UGT and ABC transporters in the gut of the 5th instar larvae of H. cunea, but showed no significant effects on the CarE activity. 【Conclusion】 Chlorogenic acid can affect the survival rate, the food utilization efficiency and the growth and development of H. cunea larvae. H. cunea larvae may adapt chlorogenic acid in food by regulating food utilization efficiency and inducing the activities of metabolic detoxification-related proteins.

Key words: Hyphantria cunea, chlorogenic acid, nutritional effect, growth and development; detoxification-related protein