›› 1997, Vol. 40 ›› Issue (1): 1-6.

• RESEARCH PAPERS •     Next Articles

YAC-BASED TRANSFER OF FIBROIN GENE FROM ANTHERAEA YAMAMAI TO BOMBYX MORI--EXPRESSION OF THE YAC CLONE CONTAINING A. YAMAMA/FIBROIN GENE IN B. MORI

Li Zhengang Zhou Congzhao Tang Hengli   

  • Online:1997-02-20 Published:1997-02-20

Abstract: This paper is the first report of the expression of Antheraea yamamai fibroingene in Bombyx mori. We have constructed an yeast artificial chromosome (YAC) clonecontaining the fibroin gene of A. yamamai, and transferred this DNA clone into the fertilized eggs of B. moti. Dot blot indicates that the fibroin gene has integrated into the genomeof B. moti. By analyzing the fibroin amino acid composition and the silk solubility, wefound that A. yamamai fibroin gene has expressed in some of the transgenic B. moti. The existence of YAC sequence in the F2 progeny of transgenic B. morisuggested that YAC is a medium for the transfer of fibroin gene. There is only one copy of A. yam. Ami firoin gene in the haploid of F2 progeny.

Key words: Bombyx mori, Antheraea yamamai, fibroin gene, yeast artificial chromosome