This paper is the first report of the expression of Antheraea yamamai fibroingene in Bombyx mori. We have constructed an yeast artificial chromosome (YAC) clonecontaining the fibroin gene of A. yamamai, and transferred this DNA clone into the fertilized eggs of B. moti. Dot blot indicates that the fibroin gene has integrated into the genomeof B. moti. By analyzing the fibroin amino acid composition and the silk solubility, wefound that A. yamamai fibroin gene has expressed in some of the transgenic B. moti. The existence of YAC sequence in the F₂ progeny of transgenic B. morisuggested that YAC is a medium for the transfer of fibroin gene. There is only one copy of A. yam. Ami firoin gene in the haploid of F₂ progeny.

AsNPV was mixed with HasNPV, HaNPV and PsNPV separately and thethree mixtures were used to infect the larvae of Helicoverpa assulta, H. arrnigera and Pseudaletia separata respectively. The nuclear polyhedrosis viruses thus obtained were called ( AsNPV+HasNPV) -H. assulta, (AsNPV+HaNPV) -H. armigera and (AsNPV+PsNPV) -P. separata respectively. The polyhedra of the NPVs were in irregular shape and varied distinctly in sizes. The biochemical charcaters of the viruses were investigated andcompared with those of HasNPV, HaNPV and PsNPV. The polyhedrin and viral structural polypeptides of the NPVs were examined by using SDS-PAGE. The polyhedrin of all NPVs had molecular weight of about 25 000 dalton. The numbers of the viral structural polypeptides of (AsNPV+HasNPV)-H. assulta, (AsNPV+HaNPV)-H. armigera and (AsNPV+PsNPV) -P. separata were 10, 14, 5 respectively, and their molecular weights were 13 500-98 000, 13 000-88 000, 18 500-52 000 dalton. The analysis of agarose electrophoretic profiles of DNAs restriction endonuclease fragments showed that they were notidentical to those of AsNPV, HasNPV, HaNPV and PsNPV. The DNAs of the viruses had changed and resulted in the changes of viral proteins and viral morphogenesis. Agrotis segetum nuclear polyhedrosis virus (AsNPV) can enhance the infection of HasNPV, HaNPV and PsNPV. The enhancement mechanism of AsNPV is worthwhile to be further investigated.

The LD50 values and the knockdown effects of deltamethrin, permethrin and DDT to Dec-R, 2C1-R and susceptible (SP) strains of house flies were investigated. The resuits indicate that the resistances of the Dec-R, 2C1-R and DDT-R to deltamethrin, permethrin and DDT were 120 912, 6 032 and 112 folds respectively in comparison with Spstrain. Significant cross-resistance to the above insecticides as found in these three resistant strains, which also demonstrated resistance to the knockdown by pyrethroid and DDT. Theparameters of synergism of PB to deltamethrin and permethrin in Dec-R and 2CI-R were determined. The relative percentages of synergism (R%S ?) from these two resistant strains were much higher than that of the SP (R%S (S)). When crossing Dec-R femals with SP males and Dec-R males with SP femals, the resistance of F₁, adults to deltamethrin decreased 16 860 and 26 319-fold respectively as compared with that of the Dec-R. The results suggested that there is a recessive gene responsible for the deltamethrin resistance in theDec-R strain. The sensitivity of the central nervous system (CNS) in the houseflies to DDT, permethrin and deltamethrin was studied with electrophysiological technique. The results show that the CNS insensitivity is an important factor of resistance to DDT andpyrethroid in the housefly, and it is also an important cause of cross-resistance among DDT and pyrethroid analogues. These results suggested that the mechanism for pyrethroid-resistance in housefly may be caused by Kdr genes.Pretreatment with 10^-7 mol/L deltamethrin and pulse-depolarization by high K+(55 mmol/L) for synaptosomes enhanced the release of³H-choline in the susceptible houseflies. But in similar experiments using synaptosomes from Dec-R house flies and pretreated with 10^-4 mol/L deltamethrin and K⁺, the enhancment of ³H-choline release was minor or absent. These results are consistent with the hypothesis that the knockdown (Dec-R) resistance is most likely due to a decreased affinity of voltagegated sodium channel for pyrethroids.

Collected from cotton fields with low level application of insecticide and rearedwith artificial diet at constant temperature, the larvae of cotton bollworm (Helicoverpa armigera Hiibner) were separately treated with fenvalerate and four other insecticides bytopical application in the laboratory. Results showed that the resistance of the larvae has respectively reached 311.0-fold (F₁₅) to fenvalerate, 10.8-fold (F₁₂) to methomyl, 3.5-fold (F₁4 to parathion-methyl, 5.2-fold (F₁₃) to phoxim, and 4.8-fold (F₁₂) to mixture ofparathion-methyl and phoxim. The R-fenvalerate strains have distinct cross-resistance to deltamethrin (41.8 fold) and cyhalothrin (28.1 fold). The results of enzymic inhibitor and activity respectively determined with methods of bioassay and biochemistry distinctly showedthat the rise of resistance to fenvalerate was mainly associated with the increasing activities of two kinds of esteraes (mixed-function oxidases and carboxylesterase) in the larvae of cotton bollworm.

The annual variation dynamics of brown planthopper (BPH) Nilaparvata lugens (Stal) biotypes was monitored by seedling bulk test, nymph survival rate test in tiUerlng stage and systematic observation in the field from 1979 to 1994. The suitable rice cultivars and resources against the current dominant population and developmental tendency of BPH biotypes were recommended. The results indicated the damage scale of BPH population in Guangzhou on the cultivar IR26 with resistant gene Bphl became higher and higher andreached the level of biotype 2 since 1992. Furthermore, the BPH biotype 2 had become the dominant population in Guangdong Province according to the tests with the population examples collected from 7 ecological localities and the verifying test from 1992 to 1994. The resistance to BPH of IR56 and IR50404 from IRRI as their resistant parent lines PTB33 and Babawee was high and stable according to the resistant tests for years. They have been recommended as the suitable and excellent resistant resources to BPH for further breeding.Twenty-three cultivars including Jing-Xian 89 resistant to the BPH biotype 1 and 2 can beused to take place of the cultivars with resistant gene Bphl in time.

(E)-β-farnesene is a main component of aphid alarm pheromone. The sensory part of an antennae of Myzus persicae to (E)-β-farnesene is located in the primary rhinaria as shown by cutting antennae experiment. The results of olfactory test showed that (E)-βfarnesene was very effective in repelling Myzus persicae and the capability of response to(E)-βfarnesene in the adult Myzus periscae is stronger than that in the young ones by EAGtest.

We have investigated the change of protein synthesis in the silkgland and fatbody of the 5th instar larvae of Bombysc mori and the regulation of Juvenile hormone analogue (JHA738) by using the method of isotopic tracing. From the early to the late stages of the fifth instar larvae, protein synthesis in the silkgland cells of the silkworm continued to increase; the synthetic activities in the middle and late stages of fifth instar were 1.6 and 2.86 times higher than that in the early stage. Two peaksdor incorporotion of ^{3H-glycine occurred during the fifth instat development, the first original peak occurred at 72 h after the 4th ecdysis and the second peak for silk protein synthesis occurred at 192 h after the fourthecdysis. The protein synthesis in fat body cells displayed pulse like fluctuation. The treatment with JHA (4μglarva) at the early and middle stages of the fifth instar larvae inhibited theprotein synthesis originally in silkgland cells and fat body cells, but stimulated the incorporation of 3H-glycine into the silk protein. The silk protein synthetic activities of JHA-injected fifth-instar-larvae were higher than those of the control animals.}

The flight capacity of adult cotton bollworms Helicoverpa armigera (Hubner)developed from the larvae collected separately from cotton, corn, peanut, mungbean and sesame fields and from those fed separately on cotton leaf, square and boll and artificial dietwere tested. The result indicated that the order of host plants and diets in relation to flight capacity of adult followed cotton(boll＞square＞leaf)/corn＞peanut＞mungbean/sesame and artificial dite. In. diet series concerning the flight energy of the adults, the effect of the best food may exceed more than two times that of the most inferior food. The flight distance increased with the body weight of the moth, and the flight capacity of moths with complementary nutrition was about two times as much as those without adult nutrition. It was foundthat the maturation process of eggs in the moths derived from cotton leaf reared larvae was significantly prolonged in comparison with that from cotton boll reared larvae. The result also showed that the flight capacity of moths was irrelevant to the density of their larvae. Since the flight capacities of moths were not improved by poor food and high density in larval stage, it was suggested that the long-distance movement of cotton bollworm is an important behavioral response of adults to other disadvantageous environmental factors.

Comparative studies of phototactic behaviour and ommatidium fine structure are made with behaviour test and microscopy on adult corn borer, Ostrinia furnacalis(Guen6e). The moths were disposed of dark adaptation in the darkroom in different time, in the night or in the day. The results are as follows. (1)The percentage of phototactic reaction of the moths which are disposed of dark adaptation in the night is much higher than that disposed in the daytime. From 5 behaviour tests with different illuminance, the percentage of the highest phototactic response is 80%, the lowest 23%, and the average 48% for the former, and 35 %, 10%and 21% respectively for the latter. (2)The distribution of the screeningpigment in the compound eye is nearly the same between the night dark adaptation and the day dark adaptation; however, the distribution area of nuclei of retinula cells is obviously different, which implies the natural rhythm of structure changes in the ommatidia of adult cornborer. The relationship between the rhythmic changes of the structure of compound eye andphototactic responses is discussed.

This paper is the result of the forensic entomological studies carried out in the suburbs of Beijing (Qingbe, 40°0'N, 116°3'E; Miyun, 400°3'N, 116°8'E; Fangshan, 39°6'N, 115°9'E). Totally 878 beetle specimens were collected and identified and they contain38 species belonging to 13 families and 26 genera. There are 11 species (40.0% of the localtotal )only found in Qinghe; 2 species (15.4 % )only in Miyun; 11 (52.4 % )only in Fangshan.The three sites show different values of species diversity: Fangshan H'=2.45, Qinghe H'= 1.72, Miyun H'=1.60.This result is potentially applicable to find Out the removing ofcorpse between different sites.

Aculeococcus was established in 1941 by Lepage for the peculiar species A. morrisoni Lepage, the material was collected from Brazil on an undetermined plant. Till the year1957, Ferris correctly assigned to the family Eriococcidae, but didn't yet know the correctposition of the family m systematic, and never found another members in the genus.

This paper reports two new species of Helina R.-D. ,collected from the eastern Kunlun-Aerjin Mountains (36°-39°020'N,87°10'-91°18'E). The type specimens are deposited in the Institute of Entomology, Shenyang Normal College.

The present paper deals with a new genus and six new species belonging to Eriophyidae from China. The length unit is micron. All the type specimens are deposited in the Department of Plant Protection, Nanjing Agricultural University.

Host selection behaviors of parasitic wasps are a comoplex and hierarchy process,which includes habitat preference, potential host location and host location. In the field,whether a parasitic wasp successfully finds its host or not is determined by many facors suchas climatic conditions, host density, chemical environment, physiological states and expriences.This paper surveys the recent progress in the study on host selection behaviors of parasiticwasps. The following aspects are included: 1. Host searching, oviposition and post-ovipositrion behaviors of the parasitoids and the cues employed, and 2. Behavioral variations of thewasps and associated factors including genotype, physiological states, experiences and learning. Prospective application of parasitic wasps in pest insect control is discussed.