›› 1997, Vol. 40 ›› Issue (1): 45-50.

• RESEARCH PAPERS •

### REGULATION OF JUVENILE HORMONE ANALOGUE ONPROTEIN SYNNTHESIS IN THE SILKGLAND AND FATBODY OF SILKWORM BOMBYX MORI

Dai Yujin Jin xiujue

• Online:1997-02-20 Published:1997-02-20

Abstract: We have investigated the change of protein synthesis in the silkgland and fatbody of the 5th instar larvae of Bombysc mori and the regulation of Juvenile hormone analogue (JHA738) by using the method of isotopic tracing. From the early to the late stages of the fifth instar larvae, protein synthesis in the silkgland cells of the silkworm continued to increase; the synthetic activities in the middle and late stages of fifth instar were 1.6 and 2.86 times higher than that in the early stage. Two peaksdor incorporotion of 3H-glycine occurred during the fifth instat development, the first original peak occurred at 72 h after the 4th ecdysis and the second peak for silk protein synthesis occurred at 192 h after the fourthecdysis. The protein synthesis in fat body cells displayed pulse like fluctuation. The treatment with JHA (4μglarva) at the early and middle stages of the fifth instar larvae inhibited theprotein synthesis originally in silkgland cells and fat body cells, but stimulated the incorporation of 3H-glycine into the silk protein. The silk protein synthetic activities of JHA-injected fifth-instar-larvae were higher than those of the control animals.