Abstract: Transfer vector p35IE1Neo containing anti-apoptotic p35 gene and neomycinresistant gene(as selection marker) was constructed. After transfecting Sf9 cells and G418 screening, cells transformed with p35IE1Neo were obtained. One clone was picked and named Sf9-35. By PCR detection, it was found that the genome of Sf9-35 cells contain p35 gene. After actinomycin D treatment and cellular DNA electrophoresis and detection of TUNEL(TdT mediated bio-dUTP nick endlabeling), Sf9-35 cells were found to be resistant to apoptosis induced by actinomycin D.

Key words: anti-apoptosis, insect cells, p35 gene