Abstract: Two near isogenic lines (NILS) of Plutella xylostella were cultivated by back crossing for 12 successive generations between its resistant and susceptible strains to dimehypo and cartap in the laboratory. DNA from NILS was compared using optimized randomly amplified polymorphic DNA (RAPD) methods.Of the 80 primers tested, 6 primers produced 1 or more special bands in NILS of dimehypo resistance (DR), but none in the susceptible strain,and 7 primers produced 1 or more special bands in NILS of cartap resistance (CR). By repeated back crossing,the gene related to resistance was displaced to susceptible genome.Therefore, it is thought that these special bands throughout the genome in this study are likely to represent 1 or more loci that influence susceptibility to dimehypo and cartap in the diamondback moth.

Key words: Plutella xylostella, dimehypo, cartap, random amplified polymorphic DNA