Abstract: Samples of Anopheles sinensis from 10 collection sites in 9 provinces of China were analyzed for genetic polymorphism using RAPD-PCR. Twenty-three RAPD loci were studied and the results were as follows: ① The percentage of polymorphic loci was 68.2%～86.4%, and the expected mean heterozygosity was 0.249～0.348, which indicated extensive genetic polymorphism in the natural populations of An.sinensis. ② The Fst and θ were caculated using 3 measures. Mean values were 0.069～0.111 with corresponding migrating rate of 2.0～3.4. It indicated that the level of gene flow was low. ③The genetic identity among the natural populations was 0.8795～0.9973 with the mean genetic distance of 0.041±0.033. The data are within the range of intraspecific variation. Cluster analysis revealed that the genetic distance did not relate to the collection sites.

Key words: Anopheles sinensis, population, molecular genetic polymorphism