Abstract: The Bostrychidae represent a highly destructive group of wood-boring and stored product beetles. We sequenced the mitochondrioal DNA gene encoding NADH dehydrogenase subunit 4(ND4) with non-invasive DNA Genotyping from 7 samples of 5 species of this family. The length of sequences was 204 bp. Sequencecomparison analysis indicated high variations in sequences of each species. The ND4 phylogenetic trees had been constructed for 5 Bostrychidae species with PAUP 3.1.1. This result was compared with the results based on the morphology of 5 species. Furthermore, the phylogenetic relationship among the species and genera that each species belongs to were discussed. The results indicated that H. aequalis of genus Heterobostrychus diverged the earliest, then did B. parallela of genus Bostrychopsis, S. anale and S.conigerum of genus Sinoxylon, X. religiosus of genus Xylothrips in turns. The two species of S. anale and S. conigerum were closely related, and they diverged last. This was basically identical with the results based on morphology.

Key words: ND4, mtDNA, phylogeny, Bostrychidae