致倦库蚊对登革Ⅱ型病毒的中肠感染屏障作用

Abstract

Abstract: The mesenteronal infection barrier to dengue 2 virus was examined in Culex pipiens quinquefasciatus. Following large dose oral infection, the viral replication was monitored with electron microscopy, and the viral nucleic acid was detected through RT-PCR at intervals in Cx. Pipiens quinquefasciatus and Aedes albopictus. The results showed that the virus was replicated rapidly in Ae. Albopictus after infecting mesenteronal epithelial cells. However, dengue 2 virus could not be detected in the same cells and other tissues of Cx. Pipiens quinquefasciatus, which indicated that the virus was unable to infect and reproduce in it and suggested that the mesenteronal barrier to dengue 2 virus infection exist in this species of mosquito.

Key words: Culex pipiens quinquefasciatus, Aedes albopictus, dengue 2 virus, mesenteronal infection barrier

XIE Chao, ZHAO Tong-Yan^*, DONG Yan-De, LU Bao-Lin. Mesenteronal infection barrier to dengue 2 virus in Culex pipiens quinquefasciatus (Diptera: Culicidae)[J]., 2002, 45(2): 160-164.

[1]	YU Ling-Yuan, WEI Ying, CHEN Xu-Min, DING Yan-Juan, HU Yao-Wen, TANG Bin, WANG Shi-Gui. Effects of heavy metal cadmium on the trehalose metabolism in Aedes albopictus (Diptera: Culicidae) larvae [J]. Acta Entomologica Sinica, 2019, 62(11): 1250-1259.
[2]	JIA Zhi-Rong, LIU Zhuan-Zhuan, WANG Xiao-Ming, Tricia WILLIAMS, LIU Pei-Wen, LI Xiao-Cong, YAN Gui-Yun, CHEN Xiao-Guang. Screening of the deltamethrin-resistant strain of Aedes albopictus (Diptera: Culicidae) and its susceptibility to dengue virus [J]. , 2018, 61(1): 11-17.
[3]	GAO Jing-Peng, CHEN Han-Ming, MA Ya-Jun. Establishment of diagnostic doses of three pyrethroid insecticides for detecting resistance in Aedes albopictus (Diptera: Culicidae) in China [J]. , 2018, 61(1): 18-24.
[4]	LUO Lan, CUI Feng. Establishment and identification of a new cell line from Culex pipiens quinquefasciatus (Diptera: Culicidae) [J]. , 2018, 61(1): 79-85.
[5]	ZENG Dong-Qin, PENG Ying-Hui, CHEN Fei-Fei, ZHANG Yun, LIU Min. Insecticidal activity of essential oil derived from horseweed Conyza canadensis (L.) Cronq. against two mosquitoes and its volatile components [J]. , 2014, 57(2): 204-211.
[6]	YUE Qiu-Juan, YAO Shu-Min, LIU Yang-Yang, LIU Shi-Juan, CUI Feng . Screening of an insecticide susceptible strain of Culex pipiens quinquefasciatus (Diptera: Culicidae) [J]. , 2013, 56(4): 379-384.
[7]	HUANG Ji-Guang, Zhao-Huan-Huan, Miao-Hui, Xu-Han-Hong. Insecticidal activities and active ingredients of Cacalia tangutica against Musca domestica and Aedes albopictus [J]. , 2012, 55(12): 1355-1361.
[8]	YAN Liang-Zhen, QIAO Chuan-Ling, ZHANG Jian-Zhen, MA En-Bo, CUI Feng. Histological observation of major structures of the mosquito Culex pipiens quinquefasciatus (Diptera: Culicidae) using paraffin tissue section [J]. , 2011, 54(3): 373-380.
[9]	LI You-Zhi, LI Guan-Hua, WEI Xiao-Yi, LIU Zhong-Hua, XU Han-Hong. Isolation and identification of insecticidal compounds from Tephrosia purpurea (Fabaceae) bark and their insecticidal activity [J]. , 2011, 54(12): 1368-1376.
[10]	WANG Zong-De, CHEN Jin-Zhu, SONG Zhan-Qian, JIANG Zhi-Kuan, HAN Zhao-Jiu, SONG Jie. Synthesis of acetal derivatives of hydroxycitronellal and their repellent activity against mosquitoes [J]. , 2010, 53(11): 1241-1247.
[11]	ZHANG Yun, PENG Y H, CHEN F F, ZENG D Q, HUANG Y, ZHONG H Y. Bioactivity and components of essential oil from Zanthoxylum ailanthoides fructification against two mosquito species [J]. , 2009, 52(9): 1028-1033.
[12]	WANG Yu-Fang, ZHUGE Hong-Xiang, ZHOU Xia, HUANG Li-Hong. Cytotoxicity and apoptosis on C6/36 cell produced by piperine [J]. , 2007, 50(5): 481-487.
[13]	GUO Xiao-Xia¹, ZHAO Tong-Yan^1, DONG Yan-De¹, JIANG Shu-Nan², LU Bao-Lin¹. Transmission of dengue 2 virus by diapausing eggs of Aedes albopictus* [J]. , 2004, 47(4): 424-428.
[14]	GE Chun-Xi, HUANG Jiong-Lie, CHEN Guan-Jin, WU Yu^*, YU Hong-Feng. Construction of transgenic vector pB［PUBnls_EGFP_prM］ and identificatio n of its integrational function in mosquito cell [J]. , 2003, 46(5): 549-552.
[15]	SONG She-Wu¹, ZHAO Tong-Yan¹,DONG Yan-De¹, JIANG Shu-Nan², LU Bao-Lin¹. Sequencing and sequence analysis of the wsp gene of Wolbachia in Chinese mosquitoes [J]. , 2002, 45(5): 571-577.

Mesenteronal infection barrier to dengue 2 virus in Culex pipiens quinquefasciatus (Diptera: Culicidae)

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10