›› 2002, Vol. 45 ›› Issue (2): 279-282.

• RESEARCH PAPERS • Previous Articles    

Establishment of a cell line from the hemocytes of Xestia c-nigrum L. (Lepidoptera: Noctuidae)

LI Chang-You1, ZHENG Gui-Ling1, WANG Xiao-Yun1, SONG Jie1, LI Guo-Xun   

  • Online:2002-04-20 Published:2002-04-20

Abstract: A new insect cell line, NEAU-Xc-960716H, was established from Xestia c-nigrum larval hemocytes through successive passage over 70 generations since July 1996. The cells were classified into two types: spherical and spindle. The population doubling time of the cell line was about 63 hours. The chromosomes were condensed short rods and round, typical in lepidopteran cell lines. The isozyme pedigree of esterase was different from the embryonic cell lines NEAU-Xc-730E of Xestia c-nigrum and IPLB-SF-21. The cell line was susceptible to Xestia c-nigrum nuclear polyhedrosis virus (XcNPV), although at a low level.

Key words: Xestia c-nigrum, cell line, hemocyte