›› 2002, Vol. 45 ›› Issue (3): 290-295.

• RESEARCH PAPERS • Previous Articles     Next Articles

Analyses of small subunit ribosomal RNA sequence of the microsporidium, Nosema bombycis and its secondary structure

WANG Jian-Yang1, HUANG Ke-Wei1, LU Chang-De2   

  • Online:2002-06-20 Published:2002-06-20

Abstract: The nucleotide sequence (1 205 bp) of small subunit ribosomal RNA (SSUrRNA) of the microsporidium, Nosema bombycis (Zhenjiang), was amplified by polymerase chain reaction(PCR). Its 3'-end was obtained using the single specific primer-PCR technique with the primers matching the highly conserved parts of SSUrRNA genes cloned. The entire coding region for SSUrRNA of N. bombycis (Zhenjiang) was 1 233 bp as described elsewhere. The sequence shared high homology with those of many microsporidia, especially N. apis. The secondary structure of the SSUrRNA was constructed with RnaViz, Forcon and DCSE. There were no helices such as 10, E10-1, 11,18, E23-n and 43 in it.

Key words: Nosema bombycis, SSP-PCR, SSUrRNA gene, secondary structure, helix