›› 2002, Vol. 45 ›› Issue (3): 318-322.

• RESEARCH PAPERS • Previous Articles     Next Articles

Effects of metal ions and urea on alkaline phosphatase from Ericerus pela (Chavannes)

ZHAO Xin-Ping, SHU Chang, YANG Fang, LIU Ke-Wu, YU Dong   

  • Online:2002-06-20 Published:2002-06-20

Abstract: The effects of metal ions and urea on the alkaline phosphatase (AKP) activity from Ericerus pela (Chavannes) were surveyed. The enzyme was purified from the female adult. After each metal ion and urea had been mixed with the enzyme, the enzyme activity was determined. K+, Na+ and Li+ had not any effect on the enzyme activity. Ca2+, Ba2+, Mg2+, Mn2+, Co2+, Ni2+ activated the enzyme. Zn2+, Cu2+, Cd2+ and Pb2+ inhibited the enzyme. The Ca2+ was a more efficacious activator and the effect was classified as noncompetitive type. The inhibition of Cu2+ on the enzyme was found to be of noncompetitive type. The AKP was inactivated by urea; the inactivation could be divided into two kinds according to the concentration of the urea: lower than 3 mol/L and higher than 3 mol/L. At lower concentration, the effect of urea on the enzyme displayed a mixed type of inhibition.

Key words: Ericerus pela (Chavannes), metal ion, urea, alkaline phosphatase, enzyme activity