›› 2004, Vol. 47 ›› Issue (6): 732-737.

• RESEARCH PAPERS • Previous Articles     Next Articles

Infection of the endosymbiont Wolbachia in population of Trichogramma evanescens in China

ZHONG Min, SHEN Zuo-Rui*   

  • Online:2004-12-20 Published:2004-12-20

Abstract: Wolbachia, belonging to α-Proteobacteria, is a commonly widespread group of intracellular symbiotic bacteria found in reproductive tissues of arthropods. The bacteria may spread in host populations through regulation of host reproduction. In this study, the presenc e of Wolbachia was detected in a natural population of Trichogramma evanescens in China based on the wsp gene cloning and PCR-RFLP analysis. Two wsp genes of Wolbachia infecting T. evanescens were amplified and se quenced, with their GenBank accession numbers as AY390279 and AY390280 Also two new Wolbachia strains were identified and named as wEvaA and wEvaB. Through constructing and analysing the phylogenic tree of Wolbachia strains in Trichogramma spp. Based on wsp gene, we found these two strains belonged to A group.

Key words: Wolbachia, intracellular symbiotic bacteria, infection, Trichogramma evanescens, wsp gene, RFLP