›› 2004, Vol. 47 ›› Issue (6): 754-759.

• RESEARCH PAPERS • Previous Articles     Next Articles

Genetic differentiation in the thiamethoxam-resistant strain of Bemisia tabaci B-biotype

ZHANG Li-Ping1,2,3, ZHANG You-Jun2*, ZHANG Wen-Ji1, XU Bao-Yun2, WU Qing-Jun2, XIAO Li-Feng2, ZHU Guo-Ren2   

  • Online:2004-12-20 Published:2004-12-20

Abstract: The genetic differentiation in the thiamethoxam-resistant strain of the B-biotype Bemisia tabaci was investigated by the method of AFLP marker. Then a dendrogram was constructed from the matrix of genetic distances using the STATISTICA software (4.5 version) and UPGMA (Unweighte d Pair-Group Method using the Arithmetic Averages). A significant genetic differentiation was found between the resistant and susceptive strains of Bemisia tabaci. Additionally, characteristic amplified DNA fragments, the fragment 150 bp for resistant strain and the fragment 315 bp for susceptive strain were identified using the EcoRⅠ+ACT/RMseRⅠ+CTG primer, which may serve to develop molecular detection of Bemisia tabaci resistance to thiamethoxam.

Key words: Bemisia tabaci, thiamethoxam, resistant strain, genetic differentiation, AFLP marker