Abstract: Using insect baculovirus as expression vector, various proteins have been produced successfully. But the frequency of recombination was very low (0.1%-1%) and it limited further application of the baculovirus expression vector system (BEVS). Many improved methods for the construction and isolation of recombinant baculo virus have been developed and are introduced here, with emphasis on linearization of baculovirus DNA and a method based on site-specific transposition of an expression cassette into a baculovirus shuttle vector propagated in E.coli.

Key words: Recombinant baculovirus, plaque assay, linearization technique, Bac-to-Bac