›› 2005, Vol. 48 ›› Issue (1): 1-6.

• RESEARCH PAPERS •     Next Articles

Screening and characterization of proteins from entomogenous fungi toxic to  Monochamus alternatus (Coleoptera: Cerambycidae)

LI Jian-Qing1,2,ZHANG Yong-An1*, ZHANG Xing-Yao1, YUAN Feng3, WANG Yu-Zhu1   

  • Online:2005-03-20 Published:2005-02-20
  • Contact: ZHANG YongAn

Abstract: Forty eight proteins were purified from six strains of entomogenous fungi. Four high toxic proteins were screened by bioassay, withMonochamus alternatus larvae as the targets. The further screening of 4 high toxic proteins showed that the toxicity of protein Bb36W-D was the highest in all the proteins. Protein Bb36W D is composed of two subunits of different sizes. Molecular weight is calculated to be 24 kD for the big subunit and 17 kD for the small subunit. Isoelectric point is 9.47 for the big subunit and 9.32 for the small subunit, respectively. Protein Bb36WD is composed of 16 ammonia acids. Concentrations of arginine, histidine and alanine are higher than others.

Key words: Entomogenous fungi, Monochamus, alternatus, toxic protein, screening, molecular weight, isoelectric point