Abstract: The pupae of Liriomyza sativae Blanchard were exposed to three plant volatiles, I.e., linalool,3-hexen-1-ol andβ-caryophyllene, respectively. The orientation ratio (OR) and average responding time (AR) of female adults emerged from the treated pupae to the treatment compounds were investigated with Y type olfactometer. There was no significant difference in OR and AR to 3-hexen-1-ol between the treatment (OR, 37.7%; AR, 21.5 s) and control (OR, 30.4%; AR, 35.0 s). Although there was no significant difference in OR to -β-aryophyllene between the treatment (46.2%) and control (42.0%), AR in the treatment (21.0 s) was significantly shorter than in the control (41.5 s). Both OR (52.9%) and AR (19.5 s) of the emerged females to linalool in the treatment were significantly different from that of the control (OR, 28.4 %; AR, 34.5 s). The further experiments with linalool indicated that the early developmental stage of pupae was susceptible to the chemical environment of pupae. After 1, 3, 5 or 7 day(s) old pupae were exposed to linalool for 48 h, only OR (54%) of females from 1 day old treatment was significantly higher than that of the control (26%), but AR of females from 1, 3, 5 day(s) old treatments were significantly shorter than that of the control. Both OR and AR of the females were also influenced by exposure time. After 2 day(s) old pupae were exposed to linalool for 24, 48, 72, 96, and 120 , only OR of the females from 72, 96, and 120 h treatment were significantly higher than that of the control. AR of the females from all treatments were significant shorter than that of the control. It is therefore inferred that the chemical environment in pupal stage could influence the olfactory orientation of adult females in L. sativae.

Key words: Liriomyza sativae, plant volatile, chemical environment, pupal stage, adult, orientation behavior