棉铃虫中肠微粒体P450的分离纯化

Abstract

Abstract:

A protocol comprising PEG8000 precipitation, high performance hydrophobic interaction chromatography (HPHIC) and high performance ion exchange chromatography (HPIEC) for separation and purification of cytochrome P450 in the cotton bollworm, Helicoverpa armigera, was introduced. PEG8000 precipitation was found suitable for pre-fractionation of the midgut microsomes P450 in H. armigera. The favorable conditions of HPHIC and HPIEC for purifying microsomal P450 were established. Following purification, four proteins with molecular weight of 58 kD, 47 kD, 56 kD and 45 kD were detected in the fractions with cytochrome P450 absorbance peak by silverstaining SDS-PAGE gel. The recovery rate of P450s was 14.3%, and the P450 purity was increased by 39-fold.

Key words: Helicoverpa armigera, cytochrome P450, PEG precipitation, high performance hydrophobic interaction chromatography, high performance ion exchange chromatography, purification

ZHENG Ming-Qi^1,2,ZHANG Wen-Ji², QIU Xing-Hui^1*, LENG Xin-Fu¹. Partial purification of microsomal P450s from midgut of the cotton bollworm, Helicoverpa armigera[J]., 2005, 48(3): 359-364.

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Partial purification of microsomal P450s from midgut of the cotton bollworm, Helicoverpa armigera

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