Abstract: By means of biological and environmental induction, multi-queen colony was organized,  and the queens could move around peacefully and give normal egg_laying in the same laying area. Furthermore, the aim of making multi-queens living through winter was achieved in the experiment, which revised the conclusion that the artificial multi-queen colony could live together only for a few months and would not survive winter. The experiment results showed that the rate of egg production of a single treated queen was similar to that of untreated single one，and the rate of egg production of threequeen colony and fivequeen colony were 222.94% and 367.09% of that of the single queen colony, respectively. The biological significance of the multi-queen colony and the application prospect of this technique were discussed.



Key words: Apis mellifera ligustica, multi-queen colony, biological induction, environmental induction, queen bee, rate of egg production