Abstract:

With the application of two-dimensional gel electrophoresis （2-DE） technology in biology science, the importance of constructing proteome map has been recognized. Protein maps of neonate larvae of four common carrionbreeding fly species were obtained using 2-DE in the range of pH 4－7. After silver stained detection of protein spots, we found that there were marked differences among these maps. Isoelectric points and molecular weight analysis indicated that 2-DE map could be used to identify neonate larvae of common carrion breeding flies which are confused in morphology.

Key words: Proteom, two-dimensional gel electrophoresis, forensic entomology, carrion-breeding flies, species identification