›› 2005, Vol. 48 ›› Issue (4): 622-626.

• RESEARCH PAPERS • Previous Articles     Next Articles

Localization of the Tpi gene on Z chromosome of the silkworm, Bombyx mori by real-time quantitative PCR

NIU Bao-Long, LU Shun-Lin, WENG Hong-Biao, SHEN Wei-Feng, MENG Zhi-Qi   

  1. Zhejiang University
  • Online:2005-09-08 Published:2005-08-20

Abstract:

The ratio of copy number between Bm Tpi and DH-PBAN, which is located on the 11th chromosome was detected with the real-time quantitative PCR technique. It was 1.0 in males and 0.5 in females. The ratio of copy number between Bm Tpi and Bm Kettin, which is located on Z chromosome, was same in males and females. This indicated that the copy number of Bm Tpi was equal to that of Bm Kettin in the silkworm genome, i.e. one copy in females and two in males. The results suggest that Bm Tpi is located on Z chromosome in the genome of silkworm. 
  

Key words: Bombyx mori, Bm Tpi, DH-PBAN, Bm Kettin, Z chromosome, real-time quantitative PCR