›› 2005, Vol. 48 ›› Issue (4): 637-642.

• RESEARCH PAPERS • Previous Articles     Next Articles

Analysis of protein patterns from embryo of silkworm Bombyx mori at later stages by two dimensional polyacrylamide gel electrophoresis

ZHONG Bo-Xiong,CHEN Jin-E,YAN Xin-Pei, XU Meng-Kui, LIANG Jian-She   

  1. Zhejiang University
  • Online:2005-09-08 Published:2005-08-20

Abstract:

Using two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and image analysis system, the changes of the proteins from later stages (after the shortening stage) of embryo of silkworm Bombyx mori were analyzed to discover the protein changing pattern during embryo development. A total of 209 specific protein spots were found in the 2D-PAGE pattern of embryos at the head thorax differentiation, reverse, tubercle appearance,head pigmentation, body pigmentation and hatch stages. Among them, the specific protein spots expressed in the head pigmentation stage and body pigmentation stage embryo contributed the largest quantity, namely 55 and 77 respectively. Similar to the changing pattern of the specific protein spots of embryo at the earlier stages, most of these specific protein spots disappeared in the embryos shortly afterwards. This suggested that those specific proteins might be related to the corresponding body characteristics in embryo development.

Key words: Bombyx mori, protein, two dimensional electrophoresis (2D-PAGE), image analysis, embryo development