Abstract:

Using three basic elements, i.e., actin 3 promoter of Bombyx mori, enhanced
 green fluorescent protein (EGFP) gene and the identified sequence of polyadenylation acid in SV40, the piggyBac transposon vector pBacA3EG was constructed. This vector was further confirmed by PCR, enzyme digestion and sequencing analysis, and the results showed that all elements had been inserted into the piggyBac vector correctly. Stronger green fluorescent was observed under the stereoscopic fluorescence microscope three days after the reconstructed vector was injected into the preblastodermic eggs. The results showed that the vector was constructed correctly and expressed in silkworm eggs. The transient expression of silkworm transgenic vector is not only the first step necessary for silkworm transgene, but also can be applied in gene function analysis, which establishes the foundation for functional studies of silkworm genome.

Key words: Silkworm, transgenesis, actin 3 promoter, piggyBac, green fluorescent protein