›› 2005, Vol. 48 ›› Issue (5): 804-809.

• RESEARCH PAPERS • Previous Articles     Next Articles

Cloning and analysis of cDNA fragments of two odorant binding proteins from the antenna of Musca domestica

ZHU Bin-Bin, JIANG Yong, NIU Chang-Ying, ZHOU Xing-Miao, LEI Chao-Liang   

  1. Huazhong Agricultural University
  • Online:2005-10-13 Published:2005-10-20

Abstract:

Two cDNA fragments of two different odorant binding protein (OBP) genes in Musca domestica were amplified with two pairs of specific primers by RT-PCR, and the lengths of them were 381 bp named MdomOBP1 (GenBank accession no.: AY730350) and 353 bp named MdomOBP2 (GenBank accession no.: AY730351) respectively. Sequencing and analysis showed that these two OBP cDNA fragments were characterized by typical conservative Cys. Deduced amino acid sequences were highly similar to that of six OBPs from Diptera, with sequence identities of 57%-88% for MdomOBP1 and 52%-91% for MdomOBP2

 

Key words: Musca domestica, antenna, odorant binding protein, gene, cloning, sequence analysis