›› 2006, Vol. 49 ›› Issue (1): 29-33.

• RESEARCH PAPERS • Previous Articles     Next Articles

Separation and characterization of vitellin of Nilaparvata lugens (Stål) (Homoptera: Delphacidae)

DAI Hua-Guo, YI Wei-Xian   

  1. Nanjing Agricultural University
  • Online:2006-03-03 Published:2006-02-20

Abstract:

The vitellin of the brown planthopper (Nilaparvata lugens) was assayed using polyacrylamide gel electrophoresis and different dyeing methods. The results indicated that the vitellin is a kind of protein containing sugar and fat. The molecular weight of the vitellin was 314 kD, consisting of three subunits with molecular weight of 148 kD, 124.5 kD and 39.6 kD respectively. Immunity experiment showed that the vitellin only existed in reproductive period of female adults of N. lugens. The vitellin of N. lugens was species-specific, and not reactive with the vitellin of the white back planthopper (Sogatella furcifera).

 

 

Key words: Nilaparvata lugens, vitellin, properties, immunity reaction