Abstract:

Insecticidal activities of two active components, Z,Z,Z-9,12,15-octadecatrienoic and Z,Z-9,12-octadecadienoic, isolated from Chinese indigenous plant，Sinacalia tangutica (Maxim.) B. Nord，against adults of Musca domestica vicina Macquart and their effects on physiological and biochemical factors of the house fly were studied by bioassay tracking. The results showed that M. domestica vicina exhibited typical exciting symptoms similar to that with neurotoxins after being treated with Z,Z,Z-9,12,15-octadecatrienoic and Z,Z-9,12-octadecadienoic at the concentration of 1 mg/mL. These two chemicals possessed poison effect on adult house flies, with LC₅₀ values 24 h after the treatment being 026 mg/mL and 0.43 mg/mL, respectively. But they showed weak contact poison against adult house flies, with the corrected mortalities 48 h after the treatment at the concentration of 1 mg/mL being 21.54% and 4.08%, respectively. These two chemicals had no obvious effect on AChE activity, but exhibited obvious effect on POD activity and the content of total glycogen, of adult house flies. Z,Z,Z-9,12,15-octadecatrienoic caused increases of Na-K-Mg-ATPase and Ca-ATPase activities while Z,Z-9,12-octadecadienoic caused a decrease of Mg-ATPase activity.

Key words: Musca domestica vicina, Sinacalia tangutica, active components, insecticidal activity, physiological and biochemical factors