Abstract:

Der fⅠ is a major allergen of Dermatophagoides farinae, which can induce the typeⅠallergy. In this study, the live mites of South China area which had been identified and cultured were picked. The total RNA was extracted. The Der fⅠ gene fragment was amplified with RT-PCR, cloned into pMD18-T vector, and sub-cloned into the expression vector pET. The recombinant plasmid pET24a-Der fⅠ so constructed was expressed through the induction of IPTG. The expressed Der fⅠprotein was purified by immobilized metal ion affinity chromatography (IMAC). Recombinant Der fⅠ protein was expressed in E. coli host strain BL21 star in the form of inclusion body, and its allergenicity was confirmed by the western blot.

Key words: Dermatophagoides farinae, allergen, expression, purification, characterization