›› 2006, Vol. 49 ›› Issue (5): 719-725.

• RESEARCH PAPERS •     Next Articles

Establishment and characterization of an embryo cell line of Bombyx mori, BmE-SWU2

PAN Min-Hui, CHEN Min, DING Yu-Bin, HONG Xi-Jun, LU Cheng   

  1. (Key Sericultural Laboratory of Agriculture Ministry, College of Sericulture and Biotechnology, Southwest University, Chongqing 400716, China)
  • Online:2006-11-08 Published:2006-10-20
  • Contact: LU Cheng

Abstract:

An embryo cell line of Bombyx mori, named BmE-SWU2, was established after one year primary culture. The cells are relatively single, short shuttle-like or round, and very small in shape. The cell line belongs to adhesive-type epithelium. The cell doubling time was 51.8 h. The ratio of duple cells was 89.76%. Thus, the cell line belongs to duple cell line. The chromosomes of BmE-SWU2 was typical of Lepidoptera, namely the chromosomes in metaphase were short pole-like or granule-like and the centromeres dispersed-type. BmE-SWU2 was more sensitive to BmNPV. The 50% tissue culture infection dose (TCID50) was 1.581×10-7.

Key words: Bombyx mori, embryo cell line, karyotype, growth curve, virus susceptivity