家蝇幼虫分泌物抗菌肽的生化特性初步研究

Abstract

Abstract: The influences of temperature, proteinases on antibacterial activity of the antibacterial peptides soaked out from living housefly (Musca domestica) larvae were investigated. Mean while, the blood clotting activity, minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and the molecular weight of these peptides were examined. The results showed that the antibacterial peptides had high heat stability, proteolytic stability and antibacterial activity but without blood clotting activity. The MIC and MBC of these peptides were 37 μg/mL and 75 μg/mL, respectively. Their molecular weight was about 10 kD.

Key words: Musca domestica, secretion, antibacterial peptides, antibacterial activity, biological characteristics

GUO Guo, WU Jian-Wei, FU Ping, ZHANG Yong, SONG Yu-Zhu, SONG Zhi-Kui. Biochemical characteristics of the antibacterial peptides in secretion of Musca domestica larvae[J]., 2006, 49(6): 918-923.

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Biochemical characteristics of the antibacterial peptides in secretion of Musca domestica larvae

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