›› 2007, Vol. 50 ›› Issue (4): 343-348.

• RESEARCH PAPERS • Previous Articles     Next Articles

Effect of the juvenile hormone analogue methoprene on multiplication of Spodoptera litura nucleopolyhedrovirus in the host hemolymph

LIU Yong-Ping, WANG Fang-Hai, SU Zhi-Jian, LI Guang-Hong, PANG Yi   

  1. (Institute of Entomology, State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, China)
  • Online:2007-05-22 Published:2007-08-20
  • Contact: LI Guang-Hong

Abstract: Juvenile hormone analogues (JHA) can promote multiplication of Spodoptera litura nucleopolyhedrovirus (SpltNPV). The effect of methoprene on synthesis of polyhedrin in the hemolymph of the 6th instar larvae of the host S. litura was studied by using SDS-PAGE and immunoblot methods. The results showed that the synthesis of polyhedrin in the host hemolymph was promoted in 2-3 days post treatment. The effect of methoprene on replication of SpltNPV-polh in hemolymph of the 6th instar larvae was investigated by real-time quantitative PCR. The results showed that replication of SpltNPV-polh was promoted significantly from the 4th day to the 5th day post methoprene treatment, during which polh copies increased to 1.22×1010 copy/mL.

 

Key words: Spodoptera litura, juvenile hormone analogue, SpltNPV, multiplication, hemolymph